clostridium_thermocellum_medium

CultureMech:002943 bacterial COMPLEX LIQUID
Original name: CLOSTRIDIUM THERMOCELLUM MEDIUM

Overview

External ontology
mediadive.medium:J595 (source link unavailable) — CLOSTRIDIUM THERMOCELLUM MEDIUM
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=595

Composition

flowchart LR
    CultureMech_002943["clostridium_thermocellum_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002943 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002943 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002943 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_002943 --> ing3_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002943 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Sodium_beta_glycerophosphate["Sodium beta-glycerophosphate"]
    CultureMech_002943 --> ing5_Sodium_beta_glycerophosphate
    CHEBI_132089["CHEBI:132089"]
    ing5_Sodium_beta_glycerophosphate -.-> CHEBI_132089
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002943 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_Glutathione["Glutathione"]
    CultureMech_002943 --> ing7_Glutathione
    CHEBI_16856["CHEBI:16856"]
    ing7_Glutathione -.-> CHEBI_16856
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002943 --> ing8_Yeast_extract
    ing9_Resazurin["Resazurin"]
    CultureMech_002943 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Cellulose["Cellulose"]
    CultureMech_002943 --> ing10_Cellulose
    CHEBI_18246["CHEBI:18246"]
    ing10_Cellulose -.-> CHEBI_18246

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.6 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KH2PO4
H2O4P.K 136.084 g/mol
1.43 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
7.2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.13 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 Sodium beta-glycerophosphate
C3H7O6P.2Na 216.037 g/mol
6 G_PER_L CHEBI:132089
sodium glycerol 2-phosphate
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.0011 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 Glutathione
C10H17N3O6S 307.328 g/mol
0.25 G_PER_L CHEBI:16856
glutathione
9 Yeast extract 4.5 G_PER_L
10 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
11 Cellulose
(C6H10O5)n.H2O 180.156 g/mol
10 G_PER_L CHEBI:18246
(1->4)-beta-D-glucan

Preparation

  1. Adjust pH to 7.0 - 7.2.
  2. Gas atmosphere: 95% N2 + 5% CO2.
  3. Comment: Cellulose can be replaced with 5.0 g/L cellobiose. In this case, cellobiose is filter-sterilized separately as 10% (w/v) solution in distilled water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.038635Z mediadive-import Imported from MediaDive Source: JCM, ID: J595
2026-03-13T06:19:16.728338Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002943
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.322834+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CLOSTRIDIUM THERMOCELLUM MEDIUM" to "clostridium_thermocellum_medium" for consistent matching
2026-06-05T07:40:54.327967+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.850217+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:52:40.011908+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.