Overview
- External ontology
- mediadive.medium:J641 (source link unavailable) — NCDC-99X MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=641
Composition
flowchart LR
CultureMech_002988["ncdc_99x_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002988 --> ing0_Yeast_extract
ing1_Resazurin["Resazurin"]
CultureMech_002988 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Arginine["Arginine"]
CultureMech_002988 --> ing2_Arginine
CHEBI_29016["CHEBI:29016"]
ing2_Arginine -.-> CHEBI_29016
ing3_Histidine["Histidine"]
CultureMech_002988 --> ing3_Histidine
CHEBI_27570["CHEBI:27570"]
ing3_Histidine -.-> CHEBI_27570
ing4_Phytone_peptone["Phytone peptone"]
CultureMech_002988 --> ing4_Phytone_peptone
ing5_Na2CO3["Na2CO3"]
CultureMech_002988 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002988 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_K2HPO4["K2HPO4"]
CultureMech_002988 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_KH2PO4["KH2PO4"]
CultureMech_002988 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9__NH4_2SO4["(NH4)2SO4"]
CultureMech_002988 --> ing9__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing9__NH4_2SO4 -.-> CHEBI_62946
ing10_NaCl["NaCl"]
CultureMech_002988 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002988 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002988 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 13.3333 G_PER_L | ||
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0133333 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 3 |
Arginine
C6H14N4O2 174.204 g/mol
|
28.1333 G_PER_L |
CHEBI:29016
arginine
|
|
| 4 |
Histidine
C6H9N3O2 155.157 g/mol
|
20.6667 G_PER_L |
CHEBI:27570
histidine
|
|
| 5 | Phytone peptone | 26.6667 G_PER_L | ||
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
53.3333 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
6.66667 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
12 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Dissolve components except Na2CO3 and L-cysteine x HCl x H2O in distilled water and bring volume to 1.0 L. Adjust pH to 7.0. Bring to a boil and cool down under a stream of a N2-CO2 (4:1, v/v) gas mixture. Add Na2CO3 and L-cysteine x HCl x H2O to the medium and dispense into culture vessels under the same gas mixture. Seal with butyl rubber stoppers and autoclave.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ncdc_99x_medium (CultureMech:002988)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.161621Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J641 |
| 2026-03-13T06:19:16.984837Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002988 |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.611676+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NCDC-99X MEDIUM" to "ncdc_99x_medium" for consistent matching |
| 2026-06-05T07:41:39.529066+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.498510+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:51.231317+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2437c0e3f25cb9b46c18435da5a5d826fd9b60bd0ecc4cfe46ee4ef05b1be5bd |