Overview
- External ontology
- mediadive.medium:J641 (source link unavailable) — NCDC-99X MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=641
Composition
flowchart LR
CultureMech_002988["ncdc_99x_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002988 --> ing0_Yeast_extract
ing1_Resazurin["Resazurin"]
CultureMech_002988 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Arginine["Arginine"]
CultureMech_002988 --> ing2_Arginine
CHEBI_29016["CHEBI:29016"]
ing2_Arginine -.-> CHEBI_29016
ing3_Histidine["Histidine"]
CultureMech_002988 --> ing3_Histidine
CHEBI_27570["CHEBI:27570"]
ing3_Histidine -.-> CHEBI_27570
ing4_Phytone_peptone["Phytone peptone"]
CultureMech_002988 --> ing4_Phytone_peptone
ing5_Na2CO3["Na2CO3"]
CultureMech_002988 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002988 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_K2HPO4["K2HPO4"]
CultureMech_002988 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_KH2PO4["KH2PO4"]
CultureMech_002988 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9__NH4_2SO4["(NH4)2SO4"]
CultureMech_002988 --> ing9__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing9__NH4_2SO4 -.-> CHEBI_62946
ing10_NaCl["NaCl"]
CultureMech_002988 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002988 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002988 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 13.3333 G_PER_L | ||
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0133333 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 3 |
Arginine
C6H14N4O2 174.204 g/mol
|
28.1333 G_PER_L |
CHEBI:29016
arginine
|
|
| 4 |
Histidine
C6H9N3O2 155.157 g/mol
|
20.6667 G_PER_L |
CHEBI:27570
histidine
|
|
| 5 | Phytone peptone | 26.6667 G_PER_L | ||
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
53.3333 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
6.66667 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
12 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Dissolve components except Na2CO3 and L-cysteine x HCl x H2O in distilled water and bring volume to 1.0 L. Adjust pH to 7.0. Bring to a boil and cool down under a stream of a N2-CO2 (4:1, v/v) gas mixture. Add Na2CO3 and L-cysteine x HCl x H2O to the medium and dispense into culture vessels under the same gas mixture. Seal with butyl rubber stoppers and autoclave.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.161621Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J641 |
| 2026-03-13T06:19:16.984837Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002988 |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.611676+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NCDC-99X MEDIUM" to "ncdc_99x_medium" for consistent matching |
| 2026-06-05T07:41:39.529066+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.498510+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |