Overview
- External ontology
- mediadive.medium:J662 (source link unavailable) — MBG-20
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=662
Composition
flowchart LR
CultureMech_003008["mbg_20"]
ing0_Peptone["Peptone"]
CultureMech_003008 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003008 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_003008 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Sea_Salt["Sea Salt"]
CultureMech_003008 --> ing3_Sea_Salt
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 16 G_PER_L | ||
| 2 | Yeast extract | 4 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Sea Salt | 40 G_PER_L |
Preparation
- Adjust pH to 7.0.
- Dissolve Sea salts in distilled water and sterilize by filtration. Separately, autoclave peptone, yeast extract and glucose solutions and add to the sterile Sea salts solution. Make the liquid medium anaerobic by repeated cycles (three times) of degassing and charging with argon.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mbg_20 (CultureMech:003008)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.219140Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J662 |
| 2026-03-13T06:19:17.117953Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003008 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.768236+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MBG-20" to "mbg_20" for consistent matching |
| 2026-06-05T07:41:13.347968+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:42.283804+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9b6d5adfac7d21a0f7ce0c244e504b5648e7ab705358cc492ae226c0401f2257 |