mbg_20

CultureMech:003008 bacterial COMPLEX LIQUID
Original name: MBG-20

Overview

External ontology
mediadive.medium:J662 (source link unavailable) — MBG-20
Applications
  • Microbial cultivation
pH
7.0
Notes
JCM Medium 662 MBG-20 lists 16.0 g Peptone, 4.0 g Yeast extract, 5.0 g Glucose, 40.0 g Sea salts (Sigma), and 1.0 L Distilled water, then adjusts pH to 7.0.

Composition

flowchart LR
    CultureMech_003008["mbg_20"]
    ing0_Peptone["Peptone"]
    CultureMech_003008 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003008 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_003008 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Sea_salts__Sigma_["Sea salts (Sigma)"]
    CultureMech_003008 --> ing3_Sea_salts__Sigma_
    ing4_Distilled_water["Distilled water"]
    CultureMech_003008 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Peptone 16.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 MediaDive J662 / JCM Medium 662 lists 16.0 g/L Peptone.
2 Yeast extract 4.0 G_PER_L FOODON:03315426
yeast extract
 MediaDive J662 / JCM Medium 662 lists 4.0 g/L Yeast extract.
3 Glucose
C6H12O6 180.156 g/mol
5.0 G_PER_L CHEBI:17234
glucose
 MediaDive J662 / JCM Medium 662 lists 5.0 g/L Glucose.
4 Sea salts (Sigma) 40.0 G_PER_L MediaDive J662 / JCM Medium 662 lists 40.0 g/L Sea salts (Sigma); this commercial sea-salt mixture is retained as an opaque complex component.
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 MediaDive J662 / JCM Medium 662 lists 1.0 L Distilled water.

Preparation

  1. Adjust pH to 7.0.
  2. Dissolve Sea salts (Sigma) in distilled water.
  3. Sterilize the Sea salts solution by filtration.
  4. Separately autoclave Peptone, Yeast extract, and Glucose solutions.
  5. Add the sterile Peptone, Yeast extract, and Glucose solutions to the sterile Sea salts solution.
  6. Make the liquid medium anaerobic by repeated cycles, three times, of degassing and charging with argon.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.219140Z mediadive-import Imported from MediaDive Source: JCM, ID: J662
2026-03-13T06:19:17.117953Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003008
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.768236+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MBG-20" to "mbg_20" for consistent matching
2026-06-05T07:41:13.347968+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m680_score15.py RESOLVED_JCM_662_MBG_20 Restored the JCM Medium 662 recipe with 1.0 L Distilled water, normalized Sea salts (Sigma), grounded the disclosed reducible nutrients, retained the Sigma sea-salt mixture as an opaque commercial component, and linked TOGO M680.