Overview
- External ontology
- mediadive.medium:J670 (source link unavailable) — SPIROCHAETA CALDARIA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=670
Composition
flowchart LR
CultureMech_003015["spirochaeta_caldaria_medium"]
ing0_Glucose["Glucose"]
CultureMech_003015 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Tryptone["Tryptone"]
CultureMech_003015 --> ing1_Tryptone
ing2_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003015 --> ing2_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing2_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing3_Resazurin["Resazurin"]
CultureMech_003015 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
CultureMech_003015 --> ing4_Trace_vitamins__see_Medium_No__197_
ing5_HCl["HCl"]
CultureMech_003015 --> ing5_HCl
CHEBI_17883["CHEBI:17883"]
ing5_HCl -.-> CHEBI_17883
ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003015 --> ing6_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
ing7_ZnCl2["ZnCl2"]
CultureMech_003015 --> ing7_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing7_ZnCl2 -.-> CHEBI_49976
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003015 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_H3BO3["H3BO3"]
CultureMech_003015 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003015 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003015 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003015 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003015 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003015 --> ing14_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003015 --> ing15_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003015 --> ing16_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
ing17_NaCl["NaCl"]
CultureMech_003015 --> ing17_NaCl
CHEBI_26710["CHEBI:26710"]
ing17_NaCl -.-> CHEBI_26710
ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003015 --> ing18_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003015 --> ing19_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
ing20_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003015 --> ing20_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing20_CaCl2_x_2_H2O -.-> CHEBI_86158
ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003015 --> ing21_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003015 --> ing22_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
ing23_AlK_SO4_2["AlK(SO4)2"]
CultureMech_003015 --> ing23_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing23_AlK_SO4_2 -.-> CHEBI_86463
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
1.89573 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Tryptone | 1.89573 G_PER_L | ||
| 3 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.236967 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000473934 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 5 | Trace vitamins (see Medium No. 197) | 10 G_PER_L | ||
| 6 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 7 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 8 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.016 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.006, 0.01] |
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.046 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
[Merged 2 duplicates: 0.036, 0.01] |
| 15 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 16 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 17 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 18 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 19 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 20 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 21 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 22 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 23 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 24 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
Preparation
- Mix ingredients and distribute into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers, and autoclave. Separately autoclave or *filter-sterilize the following solutions under a N2 atmosphere and add to the medium (per liter):
- Adjust pH of the medium to 7.0 with sterile NaOH, if necessary.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- spirochaeta_caldaria_medium (CultureMech:003015)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.237098Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J670 |
| 2026-03-13T03:09:05.921516Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:17.157986Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003015 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.812203+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIROCHAETA CALDARIA MEDIUM" to "spirochaeta_caldaria_medium" for consistent matching |
| 2026-06-05T07:41:46.251267+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.606804+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.044724+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 65cb28ab554132cbee05c0789aa9634dc91d7551b9a726463950088caf73cb87 |