spirochaeta_caldaria_medium

CultureMech:003015 bacterial COMPLEX LIQUID
Original name: SPIROCHAETA CALDARIA MEDIUM

Overview

External ontology
mediadive.medium:J670 (source link unavailable) — SPIROCHAETA CALDARIA MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=670

Composition

flowchart LR
    CultureMech_003015["spirochaeta_caldaria_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_003015 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Tryptone["Tryptone"]
    CultureMech_003015 --> ing1_Tryptone
    ing2_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003015 --> ing2_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing2_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing3_Resazurin["Resazurin"]
    CultureMech_003015 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_003015 --> ing4_Trace_vitamins__see_Medium_No__197_
    ing5_HCl["HCl"]
    CultureMech_003015 --> ing5_HCl
    CHEBI_17883["CHEBI:17883"]
    ing5_HCl -.-> CHEBI_17883
    ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003015 --> ing6_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing7_ZnCl2["ZnCl2"]
    CultureMech_003015 --> ing7_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing7_ZnCl2 -.-> CHEBI_49976
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003015 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_H3BO3["H3BO3"]
    CultureMech_003015 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003015 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003015 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003015 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003015 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003015 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003015 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003015 --> ing16_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing17_NaCl["NaCl"]
    CultureMech_003015 --> ing17_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing17_NaCl -.-> CHEBI_26710
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003015 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003015 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003015 --> ing20_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing20_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003015 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003015 --> ing22_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing23_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003015 --> ing23_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing23_AlK_SO4_2 -.-> CHEBI_86463

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
1.89573 G_PER_L CHEBI:17234
glucose
2 Tryptone 1.89573 G_PER_L
3 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.236967 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
4 Resazurin
C12H7NO4 229.191 g/mol
0.000473934 G_PER_L CHEBI:8806
Resazurin
5 Trace vitamins (see Medium No. 197) 10 G_PER_L
6 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
7 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
8 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.016 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.006, 0.01]
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.046 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 [Merged 2 duplicates: 0.036, 0.01]
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
17 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
18 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
21 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
24 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate

Preparation

  1. Mix ingredients and distribute into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers, and autoclave. Separately autoclave or *filter-sterilize the following solutions under a N2 atmosphere and add to the medium (per liter):
  2. Adjust pH of the medium to 7.0 with sterile NaOH, if necessary.
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.237098Z mediadive-import Imported from MediaDive Source: JCM, ID: J670
2026-03-13T03:09:05.921516Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:17.157986Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003015
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.812203+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SPIROCHAETA CALDARIA MEDIUM" to "spirochaeta_caldaria_medium" for consistent matching
2026-06-05T07:41:46.251267+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.606804+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.