Overview
- External ontology
- mediadive.medium:J675 (source link unavailable) — RUMINOCOCCUS ALBUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=675 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_003020["ruminococcus_albus_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_003020 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003020 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_003020 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Cellobiose["Cellobiose"]
CultureMech_003020 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Resazurin["Resazurin"]
CultureMech_003020 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_K2HPO4["K2HPO4"]
CultureMech_003020 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_KH2PO4["KH2PO4"]
CultureMech_003020 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_003020 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_NaCl["NaCl"]
CultureMech_003020 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003020 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003020 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_Isobutyric_acid["Isobutyric acid"]
CultureMech_003020 --> ing11_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing11_Isobutyric_acid -.-> CHEBI_16135
ing12_Isovaleric_acid["Isovaleric acid"]
CultureMech_003020 --> ing12_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing12_Isovaleric_acid -.-> CHEBI_28484
ing13_Tryptone["Tryptone"]
CultureMech_003020 --> ing13_Tryptone
ing14_Yeast_extract["Yeast extract"]
CultureMech_003020 --> ing14_Yeast_extract
ing15_Sodium_chloride["Sodium chloride"]
CultureMech_003020 --> ing15_Sodium_chloride
ing15_Sodium_chloride -.-> CHEBI_26710
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 5 G_PER_L | ||
| 2 | Yeast extract | 2 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
20 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 12 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
10 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 13 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
10 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 14 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 15 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 16 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Preparation
- Boil and cool under CO2. Under a gentle stream of CO2, add 4.0 g Na2CO3, 1.0 ml fatty acid mixture (see below) and 500 mg L-cysteine x HCl x H2O.
- Adjust pH to 7.0, distribute into appropriate vessels under N2 and autoclave.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J675_RUMINOCOCCUS_ALBUS_MEDIUM (CultureMech:003020)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.259008Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J675 |
| 2026-03-13T06:19:17.217953Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003020 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.876364+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMINOCOCCUS ALBUS MEDIUM" to "ruminococcus_albus_medium" for consistent matching |
| 2026-06-05T07:40:17.572352+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.063916+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:37.080773+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5d428a24c371b1f38541a6b914d45117f76ebae1624846327dd51ba8ee2e0361 |