Overview
- External ontology
- mediadive.medium:J675 (source link unavailable) — RUMINOCOCCUS ALBUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=675 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The earlier LB commercial-product expansion is superseded: the exact recipe specifies a methylbutyric acid input instead. Remove only the reviewed LB supplier nodes, preserving genuine peptone, yeast extract and salt entries. Restore the acid at its source preparation stage, without inventing a chemical ID.
Composition
flowchart LR
CultureMech_003020["ruminococcus_albus_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_003020 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003020 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_003020 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Cellobiose["Cellobiose"]
CultureMech_003020 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Resazurin["Resazurin"]
CultureMech_003020 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Distilled_water["Distilled water"]
CultureMech_003020 --> ing5_Distilled_water
ing6_Na2CO3["Na2CO3"]
CultureMech_003020 --> ing6_Na2CO3
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003020 --> ing7_L_Cysteine_HCl_x_H2O
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | Source batch input: 5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. Source qualifier: BD-Difco. | ||
| 2 | Yeast extract | Source batch input: 2 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. Source qualifier: BD-Difco. | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
CHEBI:17234
glucose
|
Source batch input: 3 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
CHEBI:17057
cellobiose
|
Source batch input: 2 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Source batch input: 1 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 6 | Distilled water | 0.92 L | Source water batch input: 920 ml = 0.92 L. This is not a measured final volume. | |
| 7 | Na2CO3 | Source batch input: 4.0 g. Added after boiling/cooling under CO2, before distribution/autoclaving. No final volume or numeric final concentration is inferred. | ||
| 8 | L-Cysteine HCl x H2O | Source batch input: 500 mg. Added after boiling/cooling under CO2, before distribution/autoclaving. No final volume or numeric final concentration is inferred. |
Preparation
- Boil and cool under CO2. Under a gentle stream of CO2, add 4.0 g Na2CO3, 1.0 ml fatty acid mixture (see below) and 500 mg L-cysteine x HCl x H2O.
- Adjust pH to 7.0, distribute into appropriate vessels under N2 and autoclave.
Solutions
S I solution 6.0 g/L
Dose: 0.04 L
| Ingredient | Concentration | Notes |
|---|---|---|
| K2HPO4 | 6 G_PER_L | Source stock batch input: 0.6 g. Retain provider-declared stock g_l=6 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Distilled water | 0.1 L | Source water batch input: 100 ml = 0.1 L. This is not a measured final volume. |
Source preparation input: 40 ml = 0.04 L per receiving batch, not grams per liter or a dose per final liter.
Mineral solution 2
Dose: 0.04 L
| Ingredient | Concentration | Notes |
|---|---|---|
| KH2PO4 | 6 G_PER_L | Source stock batch input: 0.6 g. Retain provider-declared stock g_l=6 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| (NH4)2SO4 | 20 G_PER_L | Source stock batch input: 2 g. Retain provider-declared stock g_l=20 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| NaCl | 12 G_PER_L | Source stock batch input: 1.2 g. Retain provider-declared stock g_l=12 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| MgSO4 x 7 H2O | 2.5 G_PER_L | Source stock batch input: 0.25 g. Retain provider-declared stock g_l=2.5 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| CaCl2 x 2 H2O | 1.6 G_PER_L | Source stock batch input: 0.16 g. Retain provider-declared stock g_l=1.6 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Distilled water | 0.1 L | Source water batch input: 100 ml = 0.1 L. This is not a measured final volume. |
Source preparation input: 40 ml = 0.04 L per receiving batch, not grams per liter or a dose per final liter.
Fatty acid mixture
Dose: 0.001 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Isobutyric acid | 0.01 L | Source liquid batch input: 10 ml = 0.01 L. This is not grams per liter, a dose per final liter, or a density-based mass conversion. |
| Isovaleric acid | 0.01 L | Source liquid batch input: 10 ml = 0.01 L. This is not grams per liter, a dose per final liter, or a density-based mass conversion. |
| 2-Methylbutyric acid | 0.01 L | Source liquid batch input: 10 ml = 0.01 L. This is not grams per liter, a dose per final liter, or a density-based mass conversion. |
| Distilled water | 0.07 L | Source water batch input: 70 ml = 0.07 L. This is not a measured final volume. |
Source late addition: 1.0 ml = 0.001 L per receiving batch, not a dose per final liter. The fatty-acid stock is added under CO2 before distribution/autoclaving.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.259008Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J675 |
| 2026-03-13T06:19:17.217953Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003020 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.876364+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMINOCOCCUS ALBUS MEDIUM" to "ruminococcus_albus_medium" for consistent matching |
| 2026-06-05T07:40:17.572352+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.063916+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T20:41:03.546066+00:00 | codex | RESTORED_JCM_ACID_IDENTITIES_AND_PREPARATION_SCOPES_WITH_EVIDENCE | Superseded the erroneous LB expansion, restored source-specific fatty acids, stock definitions and doses, and retained exact preparation-stage amounts with supporting snippets. Preserved genuine same-labelled ingredients, unrelated annotations and append-only history. No final volume, percent basis, density or chemical grounding inferred. |