Overview
- External ontology
- mediadive.medium:J712 (source link unavailable) — LOWENSTEIN-JENSEN MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=712
Composition
flowchart LR
CultureMech_003059["lowenstein_jensen_medium"]
ing0_L_asparagine["L-asparagine"]
CultureMech_003059 --> ing0_L_asparagine
CHEBI_17196["CHEBI:17196"]
ing0_L_asparagine -.-> CHEBI_17196
ing1_Monopotassium_phosphate["Monopotassium phosphate"]
CultureMech_003059 --> ing1_Monopotassium_phosphate
CHEBI_63036["CHEBI:63036"]
ing1_Monopotassium_phosphate -.-> CHEBI_63036
ing2_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_003059 --> ing2_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing2_Magnesium_sulfate -.-> CHEBI_32599
ing3_Magnesium_citrate["Magnesium citrate"]
CultureMech_003059 --> ing3_Magnesium_citrate
CHEBI_6637["CHEBI:6637"]
ing3_Magnesium_citrate -.-> CHEBI_6637
ing4_Potato_flour["Potato flour"]
CultureMech_003059 --> ing4_Potato_flour
ing5_Glycerol["Glycerol"]
CultureMech_003059 --> ing5_Glycerol
CHEBI_17754["CHEBI:17754"]
ing5_Glycerol -.-> CHEBI_17754
ing6_Malachite_green["Malachite green"]
CultureMech_003059 --> ing6_Malachite_green
CHEBI_72449["CHEBI:72449"]
ing6_Malachite_green -.-> CHEBI_72449
ing7_Whole_eggs["Whole eggs"]
CultureMech_003059 --> ing7_Whole_eggs
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-asparagine
C4H8N2O3 132.119 g/mol
|
3.6 G_PER_L |
CHEBI:17196
L-asparagine
|
From BD 220908 (Lowenstein-Jensen Medium); Nitrogen source for mycobacterial growth |
| 2 |
Monopotassium phosphate
H2O4P.K 136.084 g/mol
|
2.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
From BD 220908 (Lowenstein-Jensen Medium); Buffer and phosphate source |
| 3 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.24 G_PER_L |
CHEBI:32599
magnesium sulfate
|
From BD 220908 (Lowenstein-Jensen Medium); Mineral supplement |
| 4 |
Magnesium citrate
2HO.Mg 58.319 g/mol
|
0.6 G_PER_L |
CHEBI:6637
magnesium dihydroxide
|
From BD 220908 (Lowenstein-Jensen Medium); Carbon source and chelating agent |
| 5 | Potato flour | 30.0 G_PER_L |
FOODON:03307543
potato starch
|
From BD 220908 (Lowenstein-Jensen Medium); Carbohydrate source and detoxifying agent |
| 6 |
Glycerol
C3H8O3 92.094 g/mol
|
12.0 ML_PER_L |
CHEBI:17754
glycerol
|
From BD 220908 (Lowenstein-Jensen Medium); Carbon source favoring M. tuberculosis growth |
| 7 |
Malachite green
C23H25N2.Cl 364.92 g/mol
|
0.025 G_PER_L |
CHEBI:72449
malachite green
|
From BD 220908 (Lowenstein-Jensen Medium); Selective agent inhibiting contaminating bacteria |
| 8 | Whole eggs | variable VARIABLE |
FOODON:03316061
chicken egg
|
1000 mL per 600 mL base; From BD 220908 (Lowenstein-Jensen Medium); Protein source and solidifying agent via coagulation (inspissation) |
Preparation
- Use commercially available Lowenstein-Jensen medium slants (BD 220908).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- lowenstein_jensen_medium (CultureMech:003059)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.370652Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J712 |
| 2026-02-03T11:26:55.824599 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:17.486487Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003059 |
| 2026-03-17T19:45:31.200552+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LOWENSTEIN-JENSEN MEDIUM" to "lowenstein_jensen_medium" for consistent matching |
| 2026-04-04T21:06:21.196660 | expand_lowenstein_jensen | EXPANDED_COMMERCIAL_PRODUCT | Commercial product expansion: BD Lowenstein-Jensen Medium ready-to-use slants |
| 2026-05-16T08:57:37.220600+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-17T07:06:48.301612+00:00 | cleanup_residual_errors.py | CLEANUP_RESIDUAL_ERRORS | R03_fill_missing_concentration=1 |
| 2026-08-06T03:05:40.547886+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f8a67c53302e1826c9aabcadcde38b3955c1f5d583664e0d4996bb44387cca83 |