lowenstein_jensen_medium

CultureMech:003059 bacterial COMPLEX LIQUID
Original name: LOWENSTEIN-JENSEN MEDIUM

Overview

External ontology
mediadive.medium:J712 (source link unavailable) — LOWENSTEIN-JENSEN MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=712

Composition

flowchart LR
    CultureMech_003059["lowenstein_jensen_medium"]
    ing0_L_asparagine["L-asparagine"]
    CultureMech_003059 --> ing0_L_asparagine
    CHEBI_17196["CHEBI:17196"]
    ing0_L_asparagine -.-> CHEBI_17196
    ing1_Monopotassium_phosphate["Monopotassium phosphate"]
    CultureMech_003059 --> ing1_Monopotassium_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing1_Monopotassium_phosphate -.-> CHEBI_63036
    ing2_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_003059 --> ing2_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing2_Magnesium_sulfate -.-> CHEBI_32599
    ing3_Magnesium_citrate["Magnesium citrate"]
    CultureMech_003059 --> ing3_Magnesium_citrate
    CHEBI_6637["CHEBI:6637"]
    ing3_Magnesium_citrate -.-> CHEBI_6637
    ing4_Potato_flour["Potato flour"]
    CultureMech_003059 --> ing4_Potato_flour
    ing5_Glycerol["Glycerol"]
    CultureMech_003059 --> ing5_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing5_Glycerol -.-> CHEBI_17754
    ing6_Malachite_green["Malachite green"]
    CultureMech_003059 --> ing6_Malachite_green
    CHEBI_72449["CHEBI:72449"]
    ing6_Malachite_green -.-> CHEBI_72449
    ing7_Whole_eggs["Whole eggs"]
    CultureMech_003059 --> ing7_Whole_eggs

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 L-asparagine
C4H8N2O3 132.119 g/mol
3.6 G_PER_L CHEBI:17196
L-asparagine
 From BD 220908 (Lowenstein-Jensen Medium); Nitrogen source for mycobacterial growth
2 Monopotassium phosphate
H2O4P.K 136.084 g/mol
2.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 From BD 220908 (Lowenstein-Jensen Medium); Buffer and phosphate source
3 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.24 G_PER_L CHEBI:32599
magnesium sulfate
 From BD 220908 (Lowenstein-Jensen Medium); Mineral supplement
4 Magnesium citrate
2HO.Mg 58.319 g/mol
0.6 G_PER_L CHEBI:6637
magnesium dihydroxide
 From BD 220908 (Lowenstein-Jensen Medium); Carbon source and chelating agent
5 Potato flour 30.0 G_PER_L FOODON:03307543
potato starch
 From BD 220908 (Lowenstein-Jensen Medium); Carbohydrate source and detoxifying agent
6 Glycerol
C3H8O3 92.094 g/mol
12.0 ML_PER_L CHEBI:17754
glycerol
 From BD 220908 (Lowenstein-Jensen Medium); Carbon source favoring M. tuberculosis growth
7 Malachite green
C23H25N2.Cl 364.92 g/mol
0.025 G_PER_L CHEBI:72449
malachite green
 From BD 220908 (Lowenstein-Jensen Medium); Selective agent inhibiting contaminating bacteria
8 Whole eggs variable VARIABLE FOODON:03316061
chicken egg
 1000 mL per 600 mL base; From BD 220908 (Lowenstein-Jensen Medium); Protein source and solidifying agent via coagulation (inspissation)

Preparation

  1. Use commercially available Lowenstein-Jensen medium slants (BD 220908).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.370652Z mediadive-import Imported from MediaDive Source: JCM, ID: J712
2026-02-03T11:26:55.824599 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:17.486487Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003059
2026-03-17T19:45:31.200552+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LOWENSTEIN-JENSEN MEDIUM" to "lowenstein_jensen_medium" for consistent matching
2026-04-04T21:06:21.196660 expand_lowenstein_jensen EXPANDED_COMMERCIAL_PRODUCT Commercial product expansion: BD Lowenstein-Jensen Medium ready-to-use slants
2026-05-16T08:57:37.220600+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(4 keys) -> list(3 entries)
2026-05-17T07:06:48.301612+00:00 cleanup_residual_errors.py CLEANUP_RESIDUAL_ERRORS R03_fill_missing_concentration=1
2026-09-06T00:00:00-07:00 repair_jcm_reference_wrappers_score40.py LINKED_JCM_REFERENCE_WRAPPER_CHILD Added or refreshed reciprocal parent-child link for bacterial/TOGO_M735_Lowenstein-Jensen_Medium.yaml.