ravot_modified_medium_for_thermosipho_sp_g60

CultureMech:003071 bacterial COMPLEX LIQUID
Original name: RAVOT MODIFIED MEDIUM FOR THERMOSIPHO SP. G60

Overview

External ontology
mediadive.medium:J727 (source link unavailable) — RAVOT MODIFIED MEDIUM FOR THERMOSIPHO SP. G60
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'ravot_modified_medium_for_fervidobacterium_sp_r8', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J726', 'original_category': 'bacterial'}
  • {'name': 'ravot_modified_medium_for_thermoanaerovibrio_sp_r101', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J725', 'original_category': 'bacterial'}
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=727

Composition

flowchart LR
    CultureMech_003071["ravot_modified_medium_for_thermosipho_sp_g60"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_003071 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003071 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003071 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KCl["KCl"]
    CultureMech_003071 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Sodium_acetate_x_3_H2O["Sodium acetate x 3 H2O"]
    CultureMech_003071 --> ing4_Sodium_acetate_x_3_H2O
    CHEBI_32954["CHEBI:32954"]
    ing4_Sodium_acetate_x_3_H2O -.-> CHEBI_32954
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_003071 --> ing5_Yeast_extract
    ing6_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003071 --> ing6_Trypticase_peptone
    ing7_PIPES["PIPES"]
    CultureMech_003071 --> ing7_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing7_PIPES -.-> CHEBI_39033
    ing8_Sulfur["Sulfur"]
    CultureMech_003071 --> ing8_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing8_Sulfur -.-> CHEBI_26833
    ing9_Resazurin["Resazurin"]
    CultureMech_003071 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_KH2PO4["KH2PO4"]
    CultureMech_003071 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_K2HPO4["K2HPO4"]
    CultureMech_003071 --> ing11_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing11_K2HPO4 -.-> CHEBI_131527
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003071 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.981354 G_PER_L CHEBI:31206
ammonium chloride
 Copied from referenced JCM Medium 725
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.196271 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Copied from referenced JCM Medium 725
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0981354 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Copied from referenced JCM Medium 725
4 KCl
Cl.K 74.551 g/mol
0.0981354 G_PER_L CHEBI:32588
potassium chloride
 Copied from referenced JCM Medium 725
5 Sodium acetate x 3 H2O
C2H3O2.Na 82.034 g/mol
0.814524 G_PER_L CHEBI:32954
sodium acetate
 Copied from referenced JCM Medium 725
6 Yeast extract 4.90677 G_PER_L Copied from referenced JCM Medium 725
7 Trypticase peptone 4.90677 G_PER_L Copied from referenced JCM Medium 725
8 PIPES 3.38567 G_PER_L CHEBI:39033
PIPES
 Copied from referenced JCM Medium 725
9 Sulfur
S 32.067 g/mol
4.90677 G_PER_L CHEBI:26833
sulfur atom
 Copied from referenced JCM Medium 725
10 Resazurin
C12H7NO4 229.191 g/mol
0.000981354 G_PER_L CHEBI:8806
Resazurin
 Copied from referenced JCM Medium 725
11 KH2PO4
H2O4P.K 136.084 g/mol
4.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Copied from referenced JCM Medium 725
12 K2HPO4
HO4P.2K 174.174 g/mol
4.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Copied from referenced JCM Medium 725
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Copied from referenced JCM Medium 725

Preparation

  1. Use Medium No. 725 with 20.0 g/L NaCl. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:17.415152Z mediadive-import Imported from MediaDive Source: JCM, ID: J727
2026-02-03T11:26:55.872120 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:17.577719Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003071
2026-03-17T19:45:31.310903+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RAVOT MODIFIED MEDIUM FOR THERMOSIPHO SP. G60" to "ravot_modified_medium_for_thermosipho_sp_g60" for consistent matching
2026-04-04T18:49:19.962983 copy_referenced_compositions RESOLVED_REFERENCE Resolved reference by copying from ravot_modified_medium_for_thermoanaerovibrio_sp_r101 (CultureMech:003069)
2026-05-16T08:58:13.948921+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(1 entries)
2026-06-05T07:41:43.847723+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.846803+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:54.327444+00:00 recipe-merger Merged 3 duplicate recipes into canonical record Sources: ravot_modified_medium_for_fervidobacterium_sp_r8, ravot_modified_medium_for_thermoanaerovibrio_sp_r101, ravot_modified_medium_for_thermosipho_sp_g60
2026-08-06T03:05:54.327496+00:00 merge_recipes.py MERGED_RECIPES merged 3 source recipe(s) on fingerprint 3246547a833d4aee6880484f9bbbd5a1451cd42a9073f63201c32de553386d96