ravot_modified_medium_for_thermoanaerovibrio_sp_r101

CultureMech:003069 bacterial COMPLEX LIQUID
Original name: RAVOT MODIFIED MEDIUM FOR THERMOANAEROVIBRIO SP. R101

Overview

External ontology
mediadive.medium:J725 (source link unavailable) — RAVOT MODIFIED MEDIUM FOR THERMOANAEROVIBRIO SP. R101
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=725

Composition

flowchart LR
    CultureMech_003069["ravot_modified_medium_for_thermoanaerovibrio_sp_r101"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_003069 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003069 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003069 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KCl["KCl"]
    CultureMech_003069 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Sodium_acetate_x_3_H2O["Sodium acetate x 3 H2O"]
    CultureMech_003069 --> ing4_Sodium_acetate_x_3_H2O
    CHEBI_32954["CHEBI:32954"]
    ing4_Sodium_acetate_x_3_H2O -.-> CHEBI_32954
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_003069 --> ing5_Yeast_extract
    ing6_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003069 --> ing6_Trypticase_peptone
    ing7_PIPES["PIPES"]
    CultureMech_003069 --> ing7_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing7_PIPES -.-> CHEBI_39033
    ing8_Sulfur["Sulfur"]
    CultureMech_003069 --> ing8_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing8_Sulfur -.-> CHEBI_26833
    ing9_Resazurin["Resazurin"]
    CultureMech_003069 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_KH2PO4["KH2PO4"]
    CultureMech_003069 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_K2HPO4["K2HPO4"]
    CultureMech_003069 --> ing11_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing11_K2HPO4 -.-> CHEBI_131527
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003069 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.981354 G_PER_L CHEBI:31206
ammonium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.196271 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0981354 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 KCl
Cl.K 74.551 g/mol
0.0981354 G_PER_L CHEBI:32588
potassium chloride
5 Sodium acetate x 3 H2O
C2H3O2.Na 82.034 g/mol
0.814524 G_PER_L CHEBI:32954
sodium acetate
6 Yeast extract 4.90677 G_PER_L
7 Trypticase peptone 4.90677 G_PER_L
8 PIPES 3.38567 G_PER_L CHEBI:39033
PIPES
9 Sulfur
S 32.067 g/mol
4.90677 G_PER_L CHEBI:26833
sulfur atom
10 Resazurin
C12H7NO4 229.191 g/mol
0.000981354 G_PER_L CHEBI:8806
Resazurin
11 KH2PO4
H2O4P.K 136.084 g/mol
4.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
12 K2HPO4
HO4P.2K 174.174 g/mol
4.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Mix components except sulfur, adjust pH to 6.5 and autoclave under a N2 atmosphere. After cooling, add per liter the following solutions (autoclaved and store anaerobically):
  2. Readjust pH of the medium to 6.5.
  3. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing sulfur under a N2 atmosphere and seal with butyl rubber stoppers. Then, add the following solution (autoclaved and stored under a N2 atmosphere) to the medium:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.411179Z mediadive-import Imported from MediaDive Source: JCM, ID: J725
2026-03-13T06:19:17.571163Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003069
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.303025+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RAVOT MODIFIED MEDIUM FOR THERMOANAEROVIBRIO SP. R101" to "ravot_modified_medium_for_thermoanaerovibrio_sp_r101" for consistent matching
2026-06-05T07:41:43.838476+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.843301+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.