Overview
- External ontology
- mediadive.medium:J728 (source link unavailable) — DESULFOSPOROSINUS ACIDIPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=728
Composition
flowchart LR
CultureMech_003072["desulfosporosinus_acidiphilus_medium"]
ing0_K2SO4["K2SO4"]
CultureMech_003072 --> ing0_K2SO4
CHEBI_32036["CHEBI:32036"]
ing0_K2SO4 -.-> CHEBI_32036
ing1_Yeast_extract["Yeast extract"]
CultureMech_003072 --> ing1_Yeast_extract
ing2_Glycerol["Glycerol"]
CultureMech_003072 --> ing2_Glycerol
CHEBI_17754["CHEBI:17754"]
ing2_Glycerol -.-> CHEBI_17754
ing3_FeSO4["FeSO4"]
CultureMech_003072 --> ing3_FeSO4
CHEBI_75832["CHEBI:75832"]
ing3_FeSO4 -.-> CHEBI_75832
ing4_ZnSO4["ZnSO4"]
CultureMech_003072 --> ing4_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing4_ZnSO4 -.-> CHEBI_35176
ing5_Na2SO4["Na2SO4"]
CultureMech_003072 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_003072 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_KCl["KCl"]
CultureMech_003072 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003072 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_K2HPO4["K2HPO4"]
CultureMech_003072 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_003072 --> ing10_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing10_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003072 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003072 --> ing12_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
ing13_NaCl["NaCl"]
CultureMech_003072 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003072 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003072 --> ing15_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003072 --> ing16_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003072 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003072 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2["AlK(SO4)2"]
CultureMech_003072 --> ing19_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing19_AlK_SO4_2 -.-> CHEBI_86463
ing20_H3BO3["H3BO3"]
CultureMech_003072 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003072 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003072 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_003072 --> ing23__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing23__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing24_Na2SeO3["Na2SeO3"]
CultureMech_003072 --> ing24_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing24_Na2SeO3 -.-> CHEBI_48843
ing25_Na2SeO4["Na2SeO4"]
CultureMech_003072 --> ing25_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing25_Na2SeO4 -.-> CHEBI_77775
ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003072 --> ing26_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2SO4
2K.O4S 174.259 g/mol
|
0.87 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 2 | Yeast extract | 0.1 G_PER_L | ||
| 3 |
Glycerol
C3H8O3 92.094 g/mol
|
4 G_PER_L |
CHEBI:17754
glycerol
|
|
| 4 |
FeSO4
Fe.O4S 151.908 g/mol
|
10 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 5 |
ZnSO4
O4S.Zn 161.453 g/mol
|
7 G_PER_L |
CHEBI:35176
zinc sulfate
|
|
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.3 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
22.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
2.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
28.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 25.0, 3.0] |
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.7 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 13 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 17 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 25 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.0003 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 26 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.01 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 27 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.01 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix thoroughly, adjust to pH 5.5 with H2SO4 and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add the following solutions (filter-sterilized) to the medium:
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- desulfosporosinus_acidiphilus_medium (CultureMech:003072)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.416522Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J728 |
| 2026-03-13T03:09:21.122801Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:17.587067Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003072 |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.321114+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOSPOROSINUS ACIDIPHILUS MEDIUM" to "desulfosporosinus_acidiphilus_medium" for consistent matching |
| 2026-06-05T07:40:57.302679+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.707621+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:55.841171+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:31.804044+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0c10268ad3bc791cef36eb67935fc7710c949c62aa615fc10b9423e670164050 |