Overview
- External ontology
- mediadive.medium:J728 (source link unavailable) — DESULFOSPOROSINUS ACIDIPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=728
Composition
flowchart LR
CultureMech_003072["desulfosporosinus_acidiphilus_medium"]
ing0_K2SO4["K2SO4"]
CultureMech_003072 --> ing0_K2SO4
CHEBI_32036["CHEBI:32036"]
ing0_K2SO4 -.-> CHEBI_32036
ing1_Yeast_extract["Yeast extract"]
CultureMech_003072 --> ing1_Yeast_extract
ing2_Glycerol["Glycerol"]
CultureMech_003072 --> ing2_Glycerol
CHEBI_17754["CHEBI:17754"]
ing2_Glycerol -.-> CHEBI_17754
ing3_FeSO4["FeSO4"]
CultureMech_003072 --> ing3_FeSO4
CHEBI_75832["CHEBI:75832"]
ing3_FeSO4 -.-> CHEBI_75832
ing4_ZnSO4["ZnSO4"]
CultureMech_003072 --> ing4_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing4_ZnSO4 -.-> CHEBI_35176
ing5_Na2SO4["Na2SO4"]
CultureMech_003072 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_003072 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_KCl["KCl"]
CultureMech_003072 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003072 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_K2HPO4["K2HPO4"]
CultureMech_003072 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_003072 --> ing10_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing10_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003072 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003072 --> ing12_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
ing13_NaCl["NaCl"]
CultureMech_003072 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003072 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003072 --> ing15_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003072 --> ing16_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003072 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003072 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2["AlK(SO4)2"]
CultureMech_003072 --> ing19_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing19_AlK_SO4_2 -.-> CHEBI_86463
ing20_H3BO3["H3BO3"]
CultureMech_003072 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003072 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003072 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_003072 --> ing23__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing23__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing24_Na2SeO3["Na2SeO3"]
CultureMech_003072 --> ing24_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing24_Na2SeO3 -.-> CHEBI_48843
ing25_Na2SeO4["Na2SeO4"]
CultureMech_003072 --> ing25_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing25_Na2SeO4 -.-> CHEBI_77775
ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003072 --> ing26_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2SO4
2K.O4S 174.259 g/mol
|
0.87 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 2 | Yeast extract | 0.1 G_PER_L | ||
| 3 |
Glycerol
C3H8O3 92.094 g/mol
|
4 G_PER_L |
CHEBI:17754
glycerol
|
|
| 4 |
FeSO4
Fe.O4S 151.908 g/mol
|
10 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 5 |
ZnSO4
O4S.Zn 161.453 g/mol
|
7 G_PER_L |
CHEBI:35176
zinc sulfate
|
|
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.3 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
22.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
2.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
28.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 25.0, 3.0] |
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.7 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 13 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 17 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 25 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.0003 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 26 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.01 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 27 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.01 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix thoroughly, adjust to pH 5.5 with H2SO4 and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add the following solutions (filter-sterilized) to the medium:
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.416522Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J728 |
| 2026-03-13T03:09:21.122801Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:17.587067Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003072 |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.321114+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOSPOROSINUS ACIDIPHILUS MEDIUM" to "desulfosporosinus_acidiphilus_medium" for consistent matching |
| 2026-06-05T07:40:57.302679+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.707621+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:55.841171+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |