Overview
- External ontology
- mediadive.medium:J755 (source link unavailable) — MMJ-S304 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=755
Composition
flowchart LR
CultureMech_003098["mmj_s304_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003098 --> ing0_Yeast_extract
ing1_Resazurin["Resazurin"]
CultureMech_003098 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_NaHCO3["NaHCO3"]
CultureMech_003098 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003098 --> ing3_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_x_9_H2O -.-> CHEBI_76209
ing4_NaCl["NaCl"]
CultureMech_003098 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_K2HPO4["K2HPO4"]
CultureMech_003098 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003098 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_NH4Cl["NH4Cl"]
CultureMech_003098 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003098 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003098 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_KCl["KCl"]
CultureMech_003098 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003098 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003098 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003098 --> ing13_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing14_Biotin["Biotin"]
CultureMech_003098 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Folic_acid["Folic acid"]
CultureMech_003098 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003098 --> ing16_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing17_Thiamine_HCl["Thiamine HCl"]
CultureMech_003098 --> ing17_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing17_Thiamine_HCl -.-> CHEBI_49105
ing18_Riboflavin["Riboflavin"]
CultureMech_003098 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_003098 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003098 --> ing20_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_Calcium_pantothenate -.-> CHEBI_31345
ing21_Vitamin_B12["Vitamin B12"]
CultureMech_003098 --> ing21_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing21_Vitamin_B12 -.-> CHEBI_176843
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003098 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_Lipoic_acid["Lipoic acid"]
CultureMech_003098 --> ing23_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing23_Lipoic_acid -.-> CHEBI_16494
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.492126 G_PER_L | ||
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000492126 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
12.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.18 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.001 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.001 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 17 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 18 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 21 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 22 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components and adjust pH to 6.5 with NaOH. Bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following solutions (autoclaved or *filter-sterilize) from anaerobic stocks:
- Adjust pH of the medium to 6.0 - 6.5, if necessary. Pressurize the inoculated bottles to 200 kPa N2-CO2 (4:1, v/v).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mmj_s304_medium (CultureMech:003098)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.499263Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J755 |
| 2026-03-13T06:19:17.800794Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003098 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.567818+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJ-S304 MEDIUM" to "mmj_s304_medium" for consistent matching |
| 2026-06-05T07:41:35.510303+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.293260+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.228215+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ee65bfdf063d0ae6f4544e71927e9cf385ff360e35f2a339229c054834501ce6 |