mmj_s304_medium

CultureMech:003098 bacterial COMPLEX LIQUID
Original name: MMJ-S304 MEDIUM

Overview

External ontology
mediadive.medium:J755 (source link unavailable) — MMJ-S304 MEDIUM
Applications
  • Microbial cultivation
pH
6.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=755

Composition

flowchart LR
    CultureMech_003098["mmj_s304_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_003098 --> ing0_Yeast_extract
    ing1_Resazurin["Resazurin"]
    CultureMech_003098 --> ing1_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing1_Resazurin -.-> CHEBI_8806
    ing2_NaHCO3["NaHCO3"]
    CultureMech_003098 --> ing2_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing2_NaHCO3 -.-> CHEBI_32139
    ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003098 --> ing3_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing3_Na2S_x_9_H2O -.-> CHEBI_76209
    ing4_NaCl["NaCl"]
    CultureMech_003098 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_K2HPO4["K2HPO4"]
    CultureMech_003098 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003098 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_NH4Cl["NH4Cl"]
    CultureMech_003098 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003098 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003098 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_KCl["KCl"]
    CultureMech_003098 --> ing10_KCl
    CHEBI_32588["CHEBI:32588"]
    ing10_KCl -.-> CHEBI_32588
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003098 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003098 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003098 --> ing13_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing14_Biotin["Biotin"]
    CultureMech_003098 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956
    ing15_Folic_acid["Folic acid"]
    CultureMech_003098 --> ing15_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing15_Folic_acid -.-> CHEBI_27470
    ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003098 --> ing16_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing17_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003098 --> ing17_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing17_Thiamine_HCl -.-> CHEBI_49105
    ing18_Riboflavin["Riboflavin"]
    CultureMech_003098 --> ing18_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing18_Riboflavin -.-> CHEBI_17015
    ing19_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003098 --> ing19_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing19_Nicotinic_acid -.-> CHEBI_15940
    ing20_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003098 --> ing20_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing20_Calcium_pantothenate -.-> CHEBI_31345
    ing21_Vitamin_B12["Vitamin B12"]
    CultureMech_003098 --> ing21_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing21_Vitamin_B12 -.-> CHEBI_176843
    ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003098 --> ing22_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing23_Lipoic_acid["Lipoic acid"]
    CultureMech_003098 --> ing23_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing23_Lipoic_acid -.-> CHEBI_16494

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.492126 G_PER_L
2 Resazurin
C12H7NO4 229.191 g/mol
0.000492126 G_PER_L CHEBI:8806
Resazurin
3 NaHCO3
CHO3.Na 84.006 g/mol
12.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
4 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
5 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
6 K2HPO4
HO4P.2K 174.174 g/mol
0.14 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.5 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.18 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.01 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.001 G_PER_L CHEBI:34887
nickel dichloride
14 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.001 G_PER_L CHEBI:131361
disodium selenite pentahydrate
15 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
16 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
17 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
18 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
19 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
20 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
21 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
22 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
23 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
24 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components and adjust pH to 6.5 with NaOH. Bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following solutions (autoclaved or *filter-sterilize) from anaerobic stocks:
  2. Adjust pH of the medium to 6.0 - 6.5, if necessary. Pressurize the inoculated bottles to 200 kPa N2-CO2 (4:1, v/v).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.499263Z mediadive-import Imported from MediaDive Source: JCM, ID: J755
2026-03-13T06:19:17.800794Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003098
2026-03-17T06:30:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.567818+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMJ-S304 MEDIUM" to "mmj_s304_medium" for consistent matching
2026-06-05T07:41:35.510303+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.293260+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.