dethiosulfovibrio_ii_medium

CultureMech:003101 bacterial COMPLEX LIQUID
Original name: DETHIOSULFOVIBRIO II MEDIUM

Overview

External ontology
mediadive.medium:J758 (source link unavailable) — DETHIOSULFOVIBRIO II MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=758

Composition

flowchart LR
    CultureMech_003101["dethiosulfovibrio_ii_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003101 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003101 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_003101 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Trisodium_citrate_x_H2O["Trisodium citrate x H2O"]
    CultureMech_003101 --> ing3_Trisodium_citrate_x_H2O
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_003101 --> ing4_Yeast_extract
    ing5_Bacto_peptone["Bacto peptone"]
    CultureMech_003101 --> ing5_Bacto_peptone
    ing6_Resazurin["Resazurin"]
    CultureMech_003101 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003101 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_003101 --> ing8_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003101 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_HCl["HCl"]
    CultureMech_003101 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003101 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_003101 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003101 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_003101 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003101 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003101 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003101 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003101 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
19.3424 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.90135 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.967118 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 Trisodium citrate x H2O 2.90135 G_PER_L
5 Yeast extract 4.83559 G_PER_L
6 Bacto peptone 1.93424 G_PER_L
7 Resazurin
C12H7NO4 229.191 g/mol
0.000483559 G_PER_L CHEBI:8806
Resazurin
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
20 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components and autoclave under a N2 atmosphere. After cooling, add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
  2. Adjust pH of the medium to 6.7 - 6.8, if necessary.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.512320Z mediadive-import Imported from MediaDive Source: JCM, ID: J758
2026-03-13T06:19:17.833284Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003101
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.605000+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DETHIOSULFOVIBRIO II MEDIUM" to "dethiosulfovibrio_ii_medium" for consistent matching
2026-06-05T07:40:58.595732+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.340926+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:32.799536+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint e4cab85f9da07095a9af0367e833dd578c2713249b2a2b615e03e5ffe29d65a4