Overview
- External ontology
- mediadive.medium:J779 (source link unavailable) — MODIFIED HALOANAEROBIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=779
Composition
flowchart LR
CultureMech_003121["modified_haloanaerobium_medium"]
ing0_NaCl["NaCl"]
CultureMech_003121 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003121 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_KCl["KCl"]
CultureMech_003121 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Yeast_extract["Yeast extract"]
CultureMech_003121 --> ing3_Yeast_extract
ing4_Trypticase_peptone["Trypticase peptone"]
CultureMech_003121 --> ing4_Trypticase_peptone
ing5_Glucose["Glucose"]
CultureMech_003121 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Sodium_thioglycolate["Sodium thioglycolate"]
CultureMech_003121 --> ing6_Sodium_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing6_Sodium_thioglycolate -.-> CHEBI_86481
ing7_Sodium_ascorbate["Sodium ascorbate"]
CultureMech_003121 --> ing7_Sodium_ascorbate
CHEBI_113451["CHEBI:113451"]
ing7_Sodium_ascorbate -.-> CHEBI_113451
ing8_Biotin["Biotin"]
CultureMech_003121 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_Folic_acid["Folic acid"]
CultureMech_003121 --> ing9_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing9_Folic_acid -.-> CHEBI_27470
ing10_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003121 --> ing10_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing10_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing11_Thiamine_HCl["Thiamine HCl"]
CultureMech_003121 --> ing11_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing11_Thiamine_HCl -.-> CHEBI_49105
ing12_Riboflavin["Riboflavin"]
CultureMech_003121 --> ing12_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavin -.-> CHEBI_17015
ing13_Nicotinic_acid["Nicotinic acid"]
CultureMech_003121 --> ing13_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing13_Nicotinic_acid -.-> CHEBI_15940
ing14_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003121 --> ing14_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing14_Calcium_pantothenate -.-> CHEBI_31345
ing15_Vitamin_B12["Vitamin B12"]
CultureMech_003121 --> ing15_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing15_Vitamin_B12 -.-> CHEBI_176843
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003121 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17_Lipoic_acid["Lipoic acid"]
CultureMech_003121 --> ing17_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing17_Lipoic_acid -.-> CHEBI_16494
ing18_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003121 --> ing18_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing18_Nitrilotriacetic_acid -.-> CHEBI_44557
ing19_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003121 --> ing19_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing19_MnSO4_x_n_H2O -.-> CHEBI_86360
ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003121 --> ing20_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
ing21_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003121 --> ing21_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing21_CoSO4_x_7_H2O -.-> CHEBI_91244
ing22_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003121 --> ing22_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing22_CaCl2_x_2_H2O -.-> CHEBI_86158
ing23_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003121 --> ing23_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing23_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003121 --> ing24_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
ing25_AlK_SO4_2["AlK(SO4)2"]
CultureMech_003121 --> ing25_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing25_AlK_SO4_2 -.-> CHEBI_86463
ing26_H3BO3["H3BO3"]
CultureMech_003121 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003121 --> ing27_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
1072.43 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 1071.43, 1.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
128.714 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 125.714, 3.0] |
| 3 |
KCl
Cl.K 74.551 g/mol
|
14.2857 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 | Yeast extract | 142.857 G_PER_L | ||
| 5 | Trypticase peptone | 142.857 G_PER_L | ||
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
25 G_PER_L |
CHEBI:17234
glucose
|
|
| 7 |
Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
|
20 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 8 |
Sodium ascorbate
C6H7O6.Na 198.106 g/mol
|
20 G_PER_L |
CHEBI:113451
sodium ascorbate
|
|
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 10 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 11 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 12 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 13 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 14 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 15 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 16 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 18 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 19 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 20 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 21 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 22 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 23 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 24 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 25 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 26 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH 8.0 with NaOH. Autoclave under a N2 atmosphere. After cooling, add anaerobically the following solutions (autoclaved or *filter-sterilized):
- Readjust pH to 8.0, if necessary.
- Prepare just before use. Filter-sterilize.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_haloanaerobium_medium (CultureMech:003121)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.574048Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J779 |
| 2026-03-13T03:09:35.640261Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:17.970087Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003121 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.762857+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED HALOANAEROBIUM MEDIUM" to "modified_haloanaerobium_medium" for consistent matching |
| 2026-06-05T07:41:36.657211+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.653196+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:48.349821+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d1d968f0f1ba93228beed585a160186b0912d3e723e76ea61626744bd077f48b |