modified_haloanaerobium_medium

CultureMech:003121 bacterial COMPLEX LIQUID
Original name: MODIFIED HALOANAEROBIUM MEDIUM

Overview

External ontology
mediadive.medium:J779 (source link unavailable) — MODIFIED HALOANAEROBIUM MEDIUM
Applications
  • Microbial cultivation
pH
8.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=779

Composition

flowchart LR
    CultureMech_003121["modified_haloanaerobium_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003121 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003121 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_KCl["KCl"]
    CultureMech_003121 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_003121 --> ing3_Yeast_extract
    ing4_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003121 --> ing4_Trypticase_peptone
    ing5_Glucose["Glucose"]
    CultureMech_003121 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_003121 --> ing6_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing6_Sodium_thioglycolate -.-> CHEBI_86481
    ing7_Sodium_ascorbate["Sodium ascorbate"]
    CultureMech_003121 --> ing7_Sodium_ascorbate
    CHEBI_113451["CHEBI:113451"]
    ing7_Sodium_ascorbate -.-> CHEBI_113451
    ing8_Biotin["Biotin"]
    CultureMech_003121 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_Folic_acid["Folic acid"]
    CultureMech_003121 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003121 --> ing10_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing10_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing11_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003121 --> ing11_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing11_Thiamine_HCl -.-> CHEBI_49105
    ing12_Riboflavin["Riboflavin"]
    CultureMech_003121 --> ing12_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing12_Riboflavin -.-> CHEBI_17015
    ing13_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003121 --> ing13_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing13_Nicotinic_acid -.-> CHEBI_15940
    ing14_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003121 --> ing14_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing14_Calcium_pantothenate -.-> CHEBI_31345
    ing15_Vitamin_B12["Vitamin B12"]
    CultureMech_003121 --> ing15_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing15_Vitamin_B12 -.-> CHEBI_176843
    ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003121 --> ing16_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing17_Lipoic_acid["Lipoic acid"]
    CultureMech_003121 --> ing17_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing17_Lipoic_acid -.-> CHEBI_16494
    ing18_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003121 --> ing18_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing18_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing19_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003121 --> ing19_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing19_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003121 --> ing20_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing21_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003121 --> ing21_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing21_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing22_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003121 --> ing22_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing22_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing23_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003121 --> ing23_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing23_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003121 --> ing24_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing25_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003121 --> ing25_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing25_AlK_SO4_2 -.-> CHEBI_86463
    ing26_H3BO3["H3BO3"]
    CultureMech_003121 --> ing26_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing26_H3BO3 -.-> CHEBI_33118
    ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003121 --> ing27_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
1072.43 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 1071.43, 1.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
128.714 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 125.714, 3.0]
3 KCl
Cl.K 74.551 g/mol
14.2857 G_PER_L CHEBI:32588
potassium chloride
4 Yeast extract 142.857 G_PER_L
5 Trypticase peptone 142.857 G_PER_L
6 Glucose
C6H12O6 180.156 g/mol
25 G_PER_L CHEBI:17234
glucose
7 Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
20 G_PER_L CHEBI:86481
sodium thioglycolate
8 Sodium ascorbate
C6H7O6.Na 198.106 g/mol
20 G_PER_L CHEBI:113451
sodium ascorbate
9 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
10 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
11 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
12 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
13 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
14 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
15 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
16 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
17 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
18 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
19 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
20 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
21 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
22 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
23 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
24 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
25 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
26 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
27 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
28 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Add components to distilled water and bring volume to 1.0 L. Adjust pH 8.0 with NaOH. Autoclave under a N2 atmosphere. After cooling, add anaerobically the following solutions (autoclaved or *filter-sterilized):
  2. Readjust pH to 8.0, if necessary.
  3. Prepare just before use. Filter-sterilize.
  4. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.574048Z mediadive-import Imported from MediaDive Source: JCM, ID: J779
2026-03-13T03:09:35.640261Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:17.970087Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003121
2026-03-17T06:30:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.762857+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED HALOANAEROBIUM MEDIUM" to "modified_haloanaerobium_medium" for consistent matching
2026-06-05T07:41:36.657211+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.653196+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.