anaerobe_medium_no_1

CultureMech:003133 bacterial COMPLEX LIQUID
Original name: ANAEROBE MEDIUM NO. 1

Overview

External ontology
mediadive.medium:J78 (source link unavailable) — ANAEROBE MEDIUM NO. 1
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=78

Composition

flowchart LR
    CultureMech_003133["anaerobe_medium_no_1"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_003133 --> ing0_Bacto_peptone
    ing1_Beef_extract["Beef extract"]
    CultureMech_003133 --> ing1_Beef_extract
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_003133 --> ing2_Yeast_extract
    ing3_Glucose["Glucose"]
    CultureMech_003133 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_K2HPO4["K2HPO4"]
    CultureMech_003133 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_KH2PO4["KH2PO4"]
    CultureMech_003133 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_003133 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003133 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003133 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Resazurin["Resazurin"]
    CultureMech_003133 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003133 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Bacto peptone 9.79432 G_PER_L
2 Beef extract 9.79432 G_PER_L
3 Yeast extract 4.89716 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
4.89716 G_PER_L CHEBI:17234
glucose
5 K2HPO4
HO4P.2K 174.174 g/mol
0.440744 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 KH2PO4
H2O4P.K 136.084 g/mol
0.323213 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 NH4Cl
Cl.H4N 53.492 g/mol
0.979432 G_PER_L CHEBI:31206
ammonium chloride
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0979432 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Adjust pH to 7.5.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.689894Z mediadive-import Imported from MediaDive Source: JCM, ID: J78
2026-03-13T06:19:18.064286Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003133
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.869898+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ANAEROBE MEDIUM NO. 1" to "anaerobe_medium_no_1" for consistent matching
2026-06-05T07:40:48.788222+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.133773+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:23.318687+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 72f00259db2a36a114a4ad41e539f92c183d12d7e0c5c92ded5cb5e301822d91