Overview
- External ontology
- mediadive.medium:J78 (source link unavailable) — ANAEROBE MEDIUM NO. 1
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=78
Composition
flowchart LR
CultureMech_003133["anaerobe_medium_no_1"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_003133 --> ing0_Bacto_peptone
ing1_Beef_extract["Beef extract"]
CultureMech_003133 --> ing1_Beef_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_003133 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_003133 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_K2HPO4["K2HPO4"]
CultureMech_003133 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KH2PO4["KH2PO4"]
CultureMech_003133 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_003133 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003133 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003133 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Resazurin["Resazurin"]
CultureMech_003133 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003133 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 9.79432 G_PER_L | ||
| 2 | Beef extract | 9.79432 G_PER_L | ||
| 3 | Yeast extract | 4.89716 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
4.89716 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.440744 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.323213 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.979432 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0979432 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Adjust pH to 7.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.689894Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J78 |
| 2026-03-13T06:19:18.064286Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003133 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.869898+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANAEROBE MEDIUM NO. 1" to "anaerobe_medium_no_1" for consistent matching |
| 2026-06-05T07:40:48.788222+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.133773+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |