Overview
- External ontology
- mediadive.medium:J794 (source link unavailable) — METHANOBACTERIUM MEDIUM (III)
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=794
Composition
flowchart LR
CultureMech_003138["methanobacterium_medium_iii"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003138 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_003138 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003138 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003138 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaHCO3["NaHCO3"]
CultureMech_003138 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Yeast_extract["Yeast extract"]
CultureMech_003138 --> ing5_Yeast_extract
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_003138 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_Resazurin["Resazurin"]
CultureMech_003138 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003138 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003138 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Biotin["Biotin"]
CultureMech_003138 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_003138 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003138 --> ing12_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing13_Thiamine_HCl["Thiamine HCl"]
CultureMech_003138 --> ing13_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_HCl -.-> CHEBI_49105
ing14_Riboflavin["Riboflavin"]
CultureMech_003138 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Nicotinic_acid["Nicotinic acid"]
CultureMech_003138 --> ing15_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing15_Nicotinic_acid -.-> CHEBI_15940
ing16_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003138 --> ing16_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing16_Calcium_pantothenate -.-> CHEBI_31345
ing17_Vitamin_B12["Vitamin B12"]
CultureMech_003138 --> ing17_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing17_Vitamin_B12 -.-> CHEBI_176843
ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003138 --> ing18_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
ing19_Lipoic_acid["Lipoic acid"]
CultureMech_003138 --> ing19_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing19_Lipoic_acid -.-> CHEBI_16494
ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003138 --> ing20_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
ing21_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003138 --> ing21_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing21_MgSO4_x_7_H2O -.-> CHEBI_31795
ing22_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003138 --> ing22_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing22_MnSO4_x_n_H2O -.-> CHEBI_86360
ing23_NaCl["NaCl"]
CultureMech_003138 --> ing23_NaCl
CHEBI_26710["CHEBI:26710"]
ing23_NaCl -.-> CHEBI_26710
ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003138 --> ing24_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
ing25_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003138 --> ing25_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing25_CoSO4_x_7_H2O -.-> CHEBI_91244
ing26_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003138 --> ing26_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing26_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing27_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003138 --> ing27_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing27_CuSO4_x_5_H2O -.-> CHEBI_31440
ing28_AlK_SO4_2["AlK(SO4)2"]
CultureMech_003138 --> ing28_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing28_AlK_SO4_2 -.-> CHEBI_86463
ing29_H3BO3["H3BO3"]
CultureMech_003138 --> ing29_H3BO3
CHEBI_33118["CHEBI:33118"]
ing29_H3BO3 -.-> CHEBI_33118
CultureMech_003138 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.130769 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.519231 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.192308 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.241346 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.141346, 0.1] |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.40385 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 | Yeast extract | 0.192308 G_PER_L | ||
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.769231 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000961538 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 14 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 16 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 17 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 18 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 19 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 20 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 21 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 22 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 23 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 24 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 25 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 26 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 27 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 28 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 29 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 30 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 31 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components, bring to a boil for several seconds and cool down under a stream of H2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved under a N2 atmosphere):
- Pressurize the inoculated vessels to 150 kPa H2-CO2 (80:20, v/v).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanobacterium_medium_iii (CultureMech:003138)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.622948Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J794 |
| 2026-03-13T03:09:19.329376Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:18.096059Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003138 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.904840+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOBACTERIUM MEDIUM (III)" to "methanobacterium_medium_iii" for consistent matching |
| 2026-06-05T07:41:30.748540+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.676662+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:29.568527+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4905ae802d6c382c6aecc1b3c6f1777ef6aace0717f107395222b8d5fbdcb762 |