methanobacterium_medium_iii

CultureMech:003138 archaea COMPLEX LIQUID
Original name: METHANOBACTERIUM MEDIUM (III)

Overview

External ontology
mediadive.medium:J794 (source link unavailable) — METHANOBACTERIUM MEDIUM (III)
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=794

Composition

flowchart LR
    CultureMech_003138["methanobacterium_medium_iii"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003138 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_003138 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003138 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003138 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaHCO3["NaHCO3"]
    CultureMech_003138 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_003138 --> ing5_Yeast_extract
    ing6_Sodium_acetate["Sodium acetate"]
    CultureMech_003138 --> ing6_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate -.-> CHEBI_32954
    ing7_Resazurin["Resazurin"]
    CultureMech_003138 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003138 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003138 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Biotin["Biotin"]
    CultureMech_003138 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_Folic_acid["Folic acid"]
    CultureMech_003138 --> ing11_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing11_Folic_acid -.-> CHEBI_27470
    ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003138 --> ing12_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing13_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003138 --> ing13_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing13_Thiamine_HCl -.-> CHEBI_49105
    ing14_Riboflavin["Riboflavin"]
    CultureMech_003138 --> ing14_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing14_Riboflavin -.-> CHEBI_17015
    ing15_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003138 --> ing15_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing15_Nicotinic_acid -.-> CHEBI_15940
    ing16_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003138 --> ing16_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_pantothenate -.-> CHEBI_31345
    ing17_Vitamin_B12["Vitamin B12"]
    CultureMech_003138 --> ing17_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing17_Vitamin_B12 -.-> CHEBI_176843
    ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003138 --> ing18_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing19_Lipoic_acid["Lipoic acid"]
    CultureMech_003138 --> ing19_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing19_Lipoic_acid -.-> CHEBI_16494
    ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003138 --> ing20_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing21_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003138 --> ing21_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing21_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing22_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003138 --> ing22_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing22_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing23_NaCl["NaCl"]
    CultureMech_003138 --> ing23_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing23_NaCl -.-> CHEBI_26710
    ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003138 --> ing24_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing25_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003138 --> ing25_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing25_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing26_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003138 --> ing26_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing26_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing27_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003138 --> ing27_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing27_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing28_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003138 --> ing28_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing28_AlK_SO4_2 -.-> CHEBI_86463
    ing29_H3BO3["H3BO3"]
    CultureMech_003138 --> ing29_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing29_H3BO3 -.-> CHEBI_33118
    CultureMech_003138 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.130769 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.519231 G_PER_L CHEBI:31206
ammonium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.192308 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.241346 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.141346, 0.1]
5 NaHCO3
CHO3.Na 84.006 g/mol
2.40385 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 Yeast extract 0.192308 G_PER_L
7 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.769231 G_PER_L CHEBI:32954
sodium acetate
8 Resazurin
C12H7NO4 229.191 g/mol
0.000961538 G_PER_L CHEBI:8806
Resazurin
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
12 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
13 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
14 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
15 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
16 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
17 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
18 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
19 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
20 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
21 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
22 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
23 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
24 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
25 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
26 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
27 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
28 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
29 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
30 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
31 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components, bring to a boil for several seconds and cool down under a stream of H2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved under a N2 atmosphere):
  2. Pressurize the inoculated vessels to 150 kPa H2-CO2 (80:20, v/v).
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.622948Z mediadive-import Imported from MediaDive Source: JCM, ID: J794
2026-03-13T03:09:19.329376Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:18.096059Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003138
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.904840+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOBACTERIUM MEDIUM (III)" to "methanobacterium_medium_iii" for consistent matching
2026-06-05T07:41:30.748540+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.676662+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.