Overview
- External ontology
- mediadive.medium:J807 (source link unavailable) — WR MEDIUM FOR METHANOBACTERIACEAE
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=807
Composition
flowchart LR
CultureMech_003152["wr_medium_for_methanobacteriaceae"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003152 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_003152 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003152 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003152 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaHCO3["NaHCO3"]
CultureMech_003152 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Tryptone["Tryptone"]
CultureMech_003152 --> ing5_Tryptone
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_003152 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003152 --> ing7_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003152 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_Resazurin["Resazurin"]
CultureMech_003152 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003152 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_003152 --> ing11_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing11_FeCl3_x_6_H2O -.-> CHEBI_86254
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003152 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003152 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_ZnCl2["ZnCl2"]
CultureMech_003152 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003152 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_H3BO3["H3BO3"]
CultureMech_003152 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_NaCl["NaCl"]
CultureMech_003152 --> ing17_NaCl
CHEBI_26710["CHEBI:26710"]
ing17_NaCl -.-> CHEBI_26710
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003152 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003152 --> ing19_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing20_Biotin["Biotin"]
CultureMech_003152 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_Folic_acid["Folic acid"]
CultureMech_003152 --> ing21_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing21_Folic_acid -.-> CHEBI_27470
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003152 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Thiamine_HCl["Thiamine HCl"]
CultureMech_003152 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_Riboflavin["Riboflavin"]
CultureMech_003152 --> ing24_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing24_Riboflavin -.-> CHEBI_17015
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_003152 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003152 --> ing26_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_pantothenate -.-> CHEBI_31345
ing27_Vitamin_B12["Vitamin B12"]
CultureMech_003152 --> ing27_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing27_Vitamin_B12 -.-> CHEBI_176843
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003152 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29_Lipoic_acid["Lipoic acid"]
CultureMech_003152 --> ing29_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29_Lipoic_acid -.-> CHEBI_16494
CultureMech_003152 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.133203 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.523996 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.199804 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.243976 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.143976, 0.1] |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.46817 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 | Tryptone | 0.979432 G_PER_L | ||
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.803134 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.117532 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.117532 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000979432 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 12 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.35 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.024 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.025 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.12 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
[Merged 2 duplicates: 0.026, 0.004] |
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 25 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 30 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 31 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix ingredients except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, and adjust pH to 7.0. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3. Dispense the medium into suitable culture vessels (e.g., 5 ml in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave L-cysteine x HCl x H2O and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add L-cysteine x HCl x H2O and Na2S x 9H2O solutions. Readjust pH to 6.7, if necessary. Pressurize the inoculated bottles to 150 kPa H2-CO2 (80:20, v/v).
- Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
- Comment: For strain JCM 10134, incubate with a 10-20% inoculum.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- wr_medium_for_methanobacteriaceae (CultureMech:003152)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.652384Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J807 |
| 2026-03-13T03:08:44.628116Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:18.158349Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003152 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.973962+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "WR MEDIUM FOR METHANOBACTERIACEAE" to "wr_medium_for_methanobacteriaceae" for consistent matching |
| 2026-06-05T07:41:55.524279+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:14.642283+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:33.074231+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 37f4d8aedc9eafea69b1f26b02c1f6813f7871e4b711a3b54852241ccc000de6 |