wr_medium_for_methanobacteriaceae

CultureMech:003152 archaea COMPLEX LIQUID
Original name: WR MEDIUM FOR METHANOBACTERIACEAE

Overview

External ontology
mediadive.medium:J807 (source link unavailable) — WR MEDIUM FOR METHANOBACTERIACEAE
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=807

Composition

flowchart LR
    CultureMech_003152["wr_medium_for_methanobacteriaceae"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003152 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_003152 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003152 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003152 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaHCO3["NaHCO3"]
    CultureMech_003152 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Tryptone["Tryptone"]
    CultureMech_003152 --> ing5_Tryptone
    ing6_Sodium_acetate["Sodium acetate"]
    CultureMech_003152 --> ing6_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate -.-> CHEBI_32954
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003152 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003152 --> ing8_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing8_Na2S_x_9_H2O -.-> CHEBI_76209
    ing9_Resazurin["Resazurin"]
    CultureMech_003152 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003152 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_003152 --> ing11_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing11_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003152 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003152 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_ZnCl2["ZnCl2"]
    CultureMech_003152 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003152 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_H3BO3["H3BO3"]
    CultureMech_003152 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_NaCl["NaCl"]
    CultureMech_003152 --> ing17_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing17_NaCl -.-> CHEBI_26710
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003152 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003152 --> ing19_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing20_Biotin["Biotin"]
    CultureMech_003152 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Folic_acid["Folic acid"]
    CultureMech_003152 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470
    ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003152 --> ing22_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003152 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_003152 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003152 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003152 --> ing26_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_pantothenate -.-> CHEBI_31345
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_003152 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003152 --> ing28_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing29_Lipoic_acid["Lipoic acid"]
    CultureMech_003152 --> ing29_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29_Lipoic_acid -.-> CHEBI_16494
    CultureMech_003152 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.133203 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.523996 G_PER_L CHEBI:31206
ammonium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.199804 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.243976 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.143976, 0.1]
5 NaHCO3
CHO3.Na 84.006 g/mol
2.46817 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 Tryptone 0.979432 G_PER_L
7 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.803134 G_PER_L CHEBI:32954
sodium acetate
8 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.117532 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
9 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.117532 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
10 Resazurin
C12H7NO4 229.191 g/mol
0.000979432 G_PER_L CHEBI:8806
Resazurin
11 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
12 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.35 G_PER_L CHEBI:86254
iron trichloride hexahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.024 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.025 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
18 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.12 G_PER_L CHEBI:34887
nickel dichloride
20 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.03 G_PER_L CHEBI:131361
disodium selenite pentahydrate
 [Merged 2 duplicates: 0.026, 0.004]
21 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
22 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
23 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
24 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
25 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
26 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
27 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
28 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
29 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
30 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
31 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Mix ingredients except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, and adjust pH to 7.0. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3. Dispense the medium into suitable culture vessels (e.g., 5 ml in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave L-cysteine x HCl x H2O and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add L-cysteine x HCl x H2O and Na2S x 9H2O solutions. Readjust pH to 6.7, if necessary. Pressurize the inoculated bottles to 150 kPa H2-CO2 (80:20, v/v).
  2. Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
  3. Comment: For strain JCM 10134, incubate with a 10-20% inoculum.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.652384Z mediadive-import Imported from MediaDive Source: JCM, ID: J807
2026-03-13T03:08:44.628116Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:18.158349Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003152
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.973962+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "WR MEDIUM FOR METHANOBACTERIACEAE" to "wr_medium_for_methanobacteriaceae" for consistent matching
2026-06-05T07:41:55.524279+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:14.642283+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.