Overview
- External ontology
- mediadive.medium:J817 (source link unavailable) — LOW-SALT METHANOTROPHIC MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=817
Composition
flowchart LR
CultureMech_003161["low_salt_methanotrophic_medium"]
ing0_KNO3["KNO3"]
CultureMech_003161 --> ing0_KNO3
CHEBI_63043["CHEBI:63043"]
ing0_KNO3 -.-> CHEBI_63043
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003161 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003161 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KBr["KBr"]
CultureMech_003161 --> ing3_KBr
CHEBI_32030["CHEBI:32030"]
ing3_KBr -.-> CHEBI_32030
ing4_Thiamine_HCl["Thiamine HCl"]
CultureMech_003161 --> ing4_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing4_Thiamine_HCl -.-> CHEBI_49105
ing5_Nicotinic_acid["Nicotinic acid"]
CultureMech_003161 --> ing5_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing5_Nicotinic_acid -.-> CHEBI_15940
ing6_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003161 --> ing6_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing6_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing7_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003161 --> ing7_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing7_p_Aminobenzoic_acid -.-> CHEBI_30753
ing8_Riboflavin["Riboflavin"]
CultureMech_003161 --> ing8_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing8_Riboflavin -.-> CHEBI_17015
ing9_Biotin["Biotin"]
CultureMech_003161 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Vitamin_B12["Vitamin B12"]
CultureMech_003161 --> ing10_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamin_B12 -.-> CHEBI_176843
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003161 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_Na2_EDTA["Na2-EDTA"]
CultureMech_003161 --> ing12_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing12_Na2_EDTA -.-> CHEBI_64734
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003161 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_003161 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003161 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003161 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003161 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003161 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_003161 --> ing19_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing19_Fe_III__EDTA -.-> CHEBI_30729
ing20_KH2PO4["KH2PO4"]
CultureMech_003161 --> ing20_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing20_KH2PO4 -.-> CHEBI_63036
ing21_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_003161 --> ing21_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing21_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing22_NaOH["NaOH"]
CultureMech_003161 --> ing22_NaOH
CHEBI_32145["CHEBI:32145"]
ing22_NaOH -.-> CHEBI_32145
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003161 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003161 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO3
K.NO3 101.102 g/mol
|
0.0999001 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0999001 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01998 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KBr
Br.K 119.002 g/mol
|
0.0999001 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 5 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.01 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 6 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.02 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 7 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 8 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 9 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.02 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.001 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 | Vitamin B12 | 0.005 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.4 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.02 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.015 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.04 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.05 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
4.5 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
|
| 21 |
KH2PO4
H2O4P.K 136.084 g/mol
|
37.425 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 22 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
48.95 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 23 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix components and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized) to the medium:
- To complete medium, adjust pH to 6.8. Then, distribute 20 ml-aliquots of the medium into sterile 120 ml-serum bottles and seal with butyl rubber stoppers. Add 100 ml methane gas (filter-sterilized) into the head space of the culture bottle through a syringe.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- low_salt_methanotrophic_medium (CultureMech:003161)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.681496Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J817 |
| 2026-03-13T06:19:18.228029Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003161 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.056367+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LOW-SALT METHANOTROPHIC MEDIUM" to "low_salt_methanotrophic_medium" for consistent matching |
| 2026-06-05T07:41:11.402535+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.046750+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:00.580097+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:40.535815+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 082cce68dd221327fd9017cdcf26a0007339ea8a881bf60c83072e7138089114 |