low_salt_methanotrophic_medium

CultureMech:003161 bacterial DEFINED LIQUID
Original name: LOW-SALT METHANOTROPHIC MEDIUM

Overview

External ontology
mediadive.medium:J817 (source link unavailable) — LOW-SALT METHANOTROPHIC MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=817

Composition

flowchart LR
    CultureMech_003161["low_salt_methanotrophic_medium"]
    ing0_KNO3["KNO3"]
    CultureMech_003161 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003161 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003161 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KBr["KBr"]
    CultureMech_003161 --> ing3_KBr
    CHEBI_32030["CHEBI:32030"]
    ing3_KBr -.-> CHEBI_32030
    ing4_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003161 --> ing4_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing4_Thiamine_HCl -.-> CHEBI_49105
    ing5_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003161 --> ing5_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing5_Nicotinic_acid -.-> CHEBI_15940
    ing6_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003161 --> ing6_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing6_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing7_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003161 --> ing7_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing7_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing8_Riboflavin["Riboflavin"]
    CultureMech_003161 --> ing8_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing8_Riboflavin -.-> CHEBI_17015
    ing9_Biotin["Biotin"]
    CultureMech_003161 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_003161 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003161 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_Na2_EDTA["Na2-EDTA"]
    CultureMech_003161 --> ing12_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing12_Na2_EDTA -.-> CHEBI_64734
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003161 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_003161 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003161 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003161 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003161 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003161 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_003161 --> ing19_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing19_Fe_III__EDTA -.-> CHEBI_30729
    ing20_KH2PO4["KH2PO4"]
    CultureMech_003161 --> ing20_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing20_KH2PO4 -.-> CHEBI_63036
    ing21_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_003161 --> ing21_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing21_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing22_NaOH["NaOH"]
    CultureMech_003161 --> ing22_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing22_NaOH -.-> CHEBI_32145
    ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003161 --> ing23_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003161 --> ing24_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
0.0999001 G_PER_L CHEBI:63043
potassium nitrate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0999001 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.01998 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 KBr
Br.K 119.002 g/mol
0.0999001 G_PER_L CHEBI:32030
potassium bromide
5 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.01 G_PER_L CHEBI:49105
thiamine hydrochloride
6 Nicotinic acid
C6H5NO2 123.111 g/mol
0.02 G_PER_L CHEBI:15940
nicotinic acid
7 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
8 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
9 Riboflavin
C17H20N4O6 376.369 g/mol
0.02 G_PER_L CHEBI:17015
riboflavin
10 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
11 Vitamin B12 0.005 G_PER_L CHEBI:176843
vitamin B12
12 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.4 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
13 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.02 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.015 G_PER_L CHEBI:33118
boric acid
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.04 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.05 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
4.5 G_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
21 KH2PO4
H2O4P.K 136.084 g/mol
37.425 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
22 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
48.95 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
23 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
24 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
25 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Mix components and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized) to the medium:
  2. To complete medium, adjust pH to 6.8. Then, distribute 20 ml-aliquots of the medium into sterile 120 ml-serum bottles and seal with butyl rubber stoppers. Add 100 ml methane gas (filter-sterilized) into the head space of the culture bottle through a syringe.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.681496Z mediadive-import Imported from MediaDive Source: JCM, ID: J817
2026-03-13T06:19:18.228029Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003161
2026-03-17T06:29:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.056367+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LOW-SALT METHANOTROPHIC MEDIUM" to "low_salt_methanotrophic_medium" for consistent matching
2026-06-05T07:41:11.402535+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.046750+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:00.580097+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.