Overview
- External ontology
- mediadive.medium:J839 (source link unavailable) — R2A BROTH
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=839
Composition
flowchart LR
CultureMech_003183["r2a_broth"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003183 --> ing0_Yeast_extract
ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_003183 --> ing1_Proteose_peptone_no__3
ing2_Casamino_acids["Casamino acids"]
CultureMech_003183 --> ing2_Casamino_acids
ing3_Glucose["Glucose"]
CultureMech_003183 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Starch["Starch"]
CultureMech_003183 --> ing4_Starch
CHEBI_28017["CHEBI:28017"]
ing4_Starch -.-> CHEBI_28017
ing5_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_003183 --> ing5_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_Sodium_pyruvate -.-> CHEBI_50144
ing6_K2HPO4["K2HPO4"]
CultureMech_003183 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003183 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.5 G_PER_L | ||
| 2 | Proteose peptone no. 3 | 0.5 G_PER_L | ||
| 3 | Casamino acids | 0.5 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
|
| 6 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Preparation
- Adjust pH to 7.2.
- Comment: The premixed powder is available from Nihon Pharm. Co. as R2A broth 'DAIGO'.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- r2a_broth (CultureMech:003183)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.758902Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J839 |
| 2026-01-28T19:48:11.541284Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:18.415158Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003183 |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.264885+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A BROTH" to "r2a_broth" for consistent matching |
| 2026-06-05T07:41:43.595208+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.001292+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:10.768671+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.098594+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6947c052fa314e7ccbd4979c3834437bead6d41be305e2755519f7f72cbbb7a4 |