r2a_broth

CultureMech:003183 bacterial COMPLEX LIQUID
Original name: R2A BROTH

Overview

External ontology
mediadive.medium:J839 (source link unavailable) — R2A BROTH
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=839

Composition

flowchart LR
    CultureMech_003183["r2a_broth"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_003183 --> ing0_Yeast_extract
    ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_003183 --> ing1_Proteose_peptone_no__3
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_003183 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_003183 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Starch["Starch"]
    CultureMech_003183 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_003183 --> ing5_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Sodium_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_003183 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003183 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.5 G_PER_L
2 Proteose peptone no. 3 0.5 G_PER_L
3 Casamino acids 0.5 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
5 Starch 0.5 G_PER_L CHEBI:28017
starch
6 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.3 G_PER_L CHEBI:50144
sodium pyruvate
7 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate

Preparation

  1. Adjust pH to 7.2.
  2. Comment: The premixed powder is available from Nihon Pharm. Co. as R2A broth 'DAIGO'.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.758902Z mediadive-import Imported from MediaDive Source: JCM, ID: J839
2026-01-28T19:48:11.541284Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:18.415158Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003183
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.264885+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R2A BROTH" to "r2a_broth" for consistent matching
2026-06-05T07:41:43.595208+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.001292+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:10.768671+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-06T00:00:00-07:00 repair_r2a_score40.py LINKED_R2A_SCORE40_CHILDREN Added or refreshed bacterial/1_10_r2a_broth.yaml as a child of bacterial/r2a_broth.yaml.
2026-09-12T00:00:00-07:00 repair_togo_m2125_r2a_daigo_score15.py LINKED_TOGO_M2125_R2A_DAIGO_SCORE15_CHILD Added or refreshed bacterial/r2a_medium_daigo.yaml as a physical-state child of bacterial/r2a_broth.yaml.
2026-10-07T14:52:40.011908+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.