Overview
- External ontology
- mediadive.medium:J848 (source link unavailable) — MODIFIED 1/10 PYGV MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=848
Composition
flowchart LR
CultureMech_003193["modified_1_10_pygv_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_003193 --> ing0_Trypticase_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003193 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_003193 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_K2HPO4["K2HPO4"]
CultureMech_003193 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KH2PO4["KH2PO4"]
CultureMech_003193 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_003193 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_NaCl["NaCl"]
CultureMech_003193 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003193 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003193 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_Biotin["Biotin"]
CultureMech_003193 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Vitamine_B12["Vitamine B12"]
CultureMech_003193 --> ing10_Vitamine_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamine_B12 -.-> CHEBI_176843
ing11_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003193 --> ing11_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing11_p_Aminobenzoic_acid -.-> CHEBI_30753
ing12_Folic_acid["Folic acid"]
CultureMech_003193 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Thiamine_HCl["Thiamine HCl"]
CultureMech_003193 --> ing13_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_HCl -.-> CHEBI_49105
ing14_Riboflavin["Riboflavin"]
CultureMech_003193 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003193 --> ing15_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 0.86881 G_PER_L | ||
| 2 | Yeast extract | 0.434405 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
8.6881 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
4.85 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 | Vitamine B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 12 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.3 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.5 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.5 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 16 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
1.5 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Preparation
- Adjust pH to 7.0. Cultivate under a N2 (100%) atmosphere.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_1_10_pygv_medium (CultureMech:003193)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.786759Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J848 |
| 2026-03-13T06:19:18.488015Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003193 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.360731+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED 1/10 PYGV MEDIUM" to "modified_1_10_pygv_medium" for consistent matching |
| 2026-06-05T07:41:36.003397+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.449876+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.770996+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 099096305477c9bca5d048b3764da97962089c9b87235c45d8ed9acb20817fbf |