modified_1_10_pygv_medium (CultureMech:003193)

Overview

External ontology

mediadive.medium:J848 (source link unavailable) — MODIFIED 1/10 PYGV MEDIUM

Applications

Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=848

Composition

flowchart LR
    CultureMech_003193["modified_1_10_pygv_medium"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003193 --> ing0_Trypticase_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003193 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_003193 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_K2HPO4["K2HPO4"]
    CultureMech_003193 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_KH2PO4["KH2PO4"]
    CultureMech_003193 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_003193 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaCl["NaCl"]
    CultureMech_003193 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003193 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003193 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_Vitamine_B12["Vitamine B12"]
    CultureMech_003193 --> ing9_Vitamine_B12
    CHEBI_176843["CHEBI:176843"]
    ing9_Vitamine_B12 -.-> CHEBI_176843

Ingredients (10)

#	Ingredient	Concentration	Ontology
1	Trypticase peptone	0.86881 G_PER_L
2	Yeast extract	0.434405 G_PER_L
3	Glucose C6H12O6 180.156 g/mol	8.6881 G_PER_L	CHEBI:17234 glucose
4	K2HPO4 HO4P.2K 174.174 g/mol	6 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
5	KH2PO4 H2O4P.K 136.084 g/mol	6 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
6	NH4Cl Cl.H4N 53.492 g/mol	4.85 G_PER_L	CHEBI:31206 ammonium chloride
7	NaCl Cl.Na 58.443 g/mol	12 G_PER_L	CHEBI:26710 sodium chloride
8	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	1 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
9	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	1.2 G_PER_L	CHEBI:86158 calcium chloride dihydrate
10	Vitamine B12	0.1 G_PER_L	CHEBI:176843 vitamin B12

Preparation

Adjust pH to 7.0. Cultivate under a N2 (100%) atmosphere.

Solutions

100 x Vitamin mixture

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Biotin	0.1 G_PER_L
p-Aminobenzoic acid	0.3 G_PER_L
Folic acid	0.5 G_PER_L
Thiamine HCl	0.5 G_PER_L
Riboflavin	0.5 G_PER_L
Pyridoxine hydrochloride	1.5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:17.786759Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J848
2026-03-13T06:19:18.488015Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:003193
2026-03-17T06:30:05.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.360731+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MODIFIED 1/10 PYGV MEDIUM" to "modified_1_10_pygv_medium" for consistent matching
2026-06-05T07:41:36.003397+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.449876+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.461283+00:00	apply_cocktail_nesting.py	NESTED_FLATTENED_COCKTAIL	Moved 6 stock-strength component(s) out of ingredients into 1 solution(s): 6 -> '100 x Vitamin mixture' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).