mo_sedi_medium

CultureMech:003201 bacterial COMPLEX LIQUID
Original name: MO-SEDI MEDIUM

Overview

External ontology
mediadive.medium:J855 (source link unavailable) — MO-SEDI MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'mo_cfxi_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J846', 'original_category': 'bacterial'}
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=855

Composition

flowchart LR
    CultureMech_003201["mo_sedi_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003201 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KH2PO4["KH2PO4"]
    CultureMech_003201 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003201 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaHCO3["NaHCO3"]
    CultureMech_003201 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_003201 --> ing4_Yeast_extract
    ing5_Tryptone["Tryptone"]
    CultureMech_003201 --> ing5_Tryptone
    ing6_Glucose["Glucose"]
    CultureMech_003201 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_003201 --> ing7_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing7_Sodium_pyruvate -.-> CHEBI_50144
    ing8_Resazurin["Resazurin"]
    CultureMech_003201 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003201 --> ing9_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003201 --> ing10_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003201 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003201 --> ing12_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing13_Biotin["Biotin"]
    CultureMech_003201 --> ing13_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotin -.-> CHEBI_15956
    ing14_Folic_acid["Folic acid"]
    CultureMech_003201 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003201 --> ing15_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing16_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003201 --> ing16_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing16_Thiamine_HCl -.-> CHEBI_49105
    ing17_Riboflavin["Riboflavin"]
    CultureMech_003201 --> ing17_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing17_Riboflavin -.-> CHEBI_17015
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003201 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_003201 --> ing19_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing19_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_003201 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003201 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_Lipoic_acid["Lipoic acid"]
    CultureMech_003201 --> ing22_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing22_Lipoic_acid -.-> CHEBI_16494
    ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003201 --> ing23_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing24_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_003201 --> ing24_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing24_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing25_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003201 --> ing25_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing25_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing26_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003201 --> ing26_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing26_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing27_ZnCl2["ZnCl2"]
    CultureMech_003201 --> ing27_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing27_ZnCl2 -.-> CHEBI_49976
    ing28_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003201 --> ing28_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing28_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing29_H3BO3["H3BO3"]
    CultureMech_003201 --> ing29_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing29_H3BO3 -.-> CHEBI_33118
    CultureMech_003201 --> more["...4 more"]

Ingredients (34)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
27.6549 G_PER_L CHEBI:26710
sodium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.110619 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.591814 G_PER_L CHEBI:31206
ammonium chloride
4 NaHCO3
CHO3.Na 84.006 g/mol
2.21239 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 Yeast extract 1.10619 G_PER_L
6 Tryptone 1.10619 G_PER_L
7 Glucose
C6H12O6 180.156 g/mol
1.99115 G_PER_L CHEBI:17234
glucose
8 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.608407 G_PER_L CHEBI:50144
sodium pyruvate
9 Resazurin
C12H7NO4 229.191 g/mol
0.00110619 G_PER_L CHEBI:8806
Resazurin
10 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
8.43333 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 8.33333, 0.1]
11 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
33.3333 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
14 Biotin
C10H16N2O3S 244.316 g/mol
0.0049 G_PER_L CHEBI:15956
biotin
15 Folic acid
C19H19N7O6 441.404 g/mol
0.0088 G_PER_L CHEBI:27470
folic acid
16 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.0041 G_PER_L CHEBI:30961
pyridoxine hydrochloride
17 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.0067 G_PER_L CHEBI:49105
thiamine hydrochloride
18 Riboflavin
C17H20N4O6 376.369 g/mol
0.0075 G_PER_L CHEBI:17015
riboflavin
19 Nicotinic acid
C6H5NO2 123.111 g/mol
0.0025 G_PER_L CHEBI:15940
nicotinic acid
20 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.0095 G_PER_L CHEBI:31345
Calcium pantothenate
21 Vitamin B12 0.0271 G_PER_L CHEBI:176843
vitamin B12
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.0027 G_PER_L CHEBI:30753
4-aminobenzoic acid
23 Lipoic acid
C8H14O2S2 206.332 g/mol
0.0041 G_PER_L CHEBI:16494
lipoic acid
24 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
25 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.35 G_PER_L CHEBI:86254
iron trichloride hexahydrate
26 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
27 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.024 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
28 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
29 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.025 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
30 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
31 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
32 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.12 G_PER_L CHEBI:34887
nickel dichloride
33 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.004 G_PER_L CHEBI:131361
disodium selenite pentahydrate
34 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Use Medium No. 846 but omit glucose and tryptone. Supplement the medium with final 2.75 g/L sodium pyruvate. Otherwise, prepare the medium as described in the recipe of Medium No. 846.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:17.809186Z mediadive-import Imported from MediaDive Source: JCM, ID: J855
2026-02-03T11:26:56.369130 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:18.541055Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003201
2026-03-17T19:45:32.420713+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MO-SEDI MEDIUM" to "mo_sedi_medium" for consistent matching
2026-04-04T18:49:20.091883 copy_referenced_compositions RESOLVED_REFERENCE Resolved reference by copying from mo_cfxi_medium (CultureMech:003191)
2026-05-16T08:58:09.094261+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(1 entries)
2026-06-05T07:41:35.894618+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.413342+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:37.365389+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: JCM_J846_MO-CFXI_MEDIUM, mo_sedi_medium
2026-08-06T03:04:37.365432+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 74544275f75eafd80d6d3367eb39c18bf4c93ebe1462510b526d9c6e974d2ab1