Overview
- External ontology
- mediadive.medium:J846 (source link unavailable) — MO-CFXI MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=846
Composition
flowchart LR
CultureMech_003191["mo_cfxi_medium"]
ing0_NaCl["NaCl"]
CultureMech_003191 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KH2PO4["KH2PO4"]
CultureMech_003191 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_003191 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaHCO3["NaHCO3"]
CultureMech_003191 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Yeast_extract["Yeast extract"]
CultureMech_003191 --> ing4_Yeast_extract
ing5_Tryptone["Tryptone"]
CultureMech_003191 --> ing5_Tryptone
ing6_Glucose["Glucose"]
CultureMech_003191 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_003191 --> ing7_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Sodium_pyruvate -.-> CHEBI_50144
ing8_Resazurin["Resazurin"]
CultureMech_003191 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003191 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003191 --> ing10_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003191 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003191 --> ing12_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing13_Biotin["Biotin"]
CultureMech_003191 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
ing14_Folic_acid["Folic acid"]
CultureMech_003191 --> ing14_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing14_Folic_acid -.-> CHEBI_27470
ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003191 --> ing15_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing16_Thiamine_HCl["Thiamine HCl"]
CultureMech_003191 --> ing16_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing16_Thiamine_HCl -.-> CHEBI_49105
ing17_Riboflavin["Riboflavin"]
CultureMech_003191 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_003191 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_003191 --> ing19_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing19_DL_Calcium_pantothenate -.-> CHEBI_31345
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_003191 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003191 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Lipoic_acid["Lipoic acid"]
CultureMech_003191 --> ing22_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing22_Lipoic_acid -.-> CHEBI_16494
ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003191 --> ing23_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
ing24_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_003191 --> ing24_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing24_FeCl3_x_6_H2O -.-> CHEBI_86254
ing25_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003191 --> ing25_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing25_MnCl2_x_4_H2O -.-> CHEBI_86368
ing26_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003191 --> ing26_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing26_CoCl2_x_6_H2O -.-> CHEBI_53503
ing27_ZnCl2["ZnCl2"]
CultureMech_003191 --> ing27_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing27_ZnCl2 -.-> CHEBI_49976
ing28_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003191 --> ing28_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing28_CuCl2_x_2_H2O -.-> CHEBI_86318
ing29_H3BO3["H3BO3"]
CultureMech_003191 --> ing29_H3BO3
CHEBI_33118["CHEBI:33118"]
ing29_H3BO3 -.-> CHEBI_33118
CultureMech_003191 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
27.6549 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.110619 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.591814 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.21239 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 | Yeast extract | 1.10619 G_PER_L | ||
| 6 | Tryptone | 1.10619 G_PER_L | ||
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
1.99115 G_PER_L |
CHEBI:17234
glucose
|
|
| 8 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.608407 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00110619 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
8.43333 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 8.33333, 0.1] |
| 11 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
33.3333 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0049 G_PER_L |
CHEBI:15956
biotin
|
|
| 15 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.0088 G_PER_L |
CHEBI:27470
folic acid
|
|
| 16 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.0041 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 17 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.0067 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.0075 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.0025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 20 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.0095 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 21 | Vitamin B12 | 0.0271 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.0027 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.0041 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 24 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 25 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.35 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 26 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 27 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.024 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 28 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 29 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.025 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 30 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 31 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 32 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.12 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 33 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.004 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 34 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix components and adjust pH to 7.0. Autoclave under a N2-CO2 (80:20, v/v) gas mixture and stand for overnight.
- Mix components and autoclave under a N2 atmosphere.
- To complete the medium, mix Solutions A and B aseptically and anaerobically under a N2-CO2 (80:20, v/v) gas mixture. Distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml medium in 50 ml serum bottle) and seal with butyl rubber stoppers. Finally add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
- Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.776775Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J846 |
| 2026-03-13T03:09:39.406319Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:18.466925Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003191 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.336833+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MO-CFXI MEDIUM" to "mo_cfxi_medium" for consistent matching |
| 2026-06-05T07:40:17.770389+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.127191+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |