Overview
- External ontology
- mediadive.medium:J891 (source link unavailable) — RAE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 3.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=891
Composition
flowchart LR
CultureMech_003240["rae_medium"]
ing0_Glucose["Glucose"]
CultureMech_003240 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Peptone["Peptone"]
CultureMech_003240 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_003240 --> ing2_Yeast_extract
ing3_Citric_acid["Citric acid"]
CultureMech_003240 --> ing3_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing3_Citric_acid -.-> CHEBI_30769
ing4_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_003240 --> ing4_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing4_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing5_Glacial_acetic_acid["Glacial acetic acid"]
CultureMech_003240 --> ing5_Glacial_acetic_acid
ing6_Ethanol["Ethanol"]
CultureMech_003240 --> ing6_Ethanol
CHEBI_16236["CHEBI:16236"]
ing6_Ethanol -.-> CHEBI_16236
ing7_Agar["Agar"]
CultureMech_003240 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
40 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Peptone | 10 G_PER_L | ||
| 3 | Yeast extract | 10 G_PER_L | ||
| 4 |
Citric acid
C6H8O7 192.123 g/mol
|
1.5 G_PER_L |
CHEBI:30769
citric acid
|
|
| 5 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
3.38 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 6 | Glacial acetic acid | 10 G_PER_L | ||
| 7 |
Ethanol
C2H6O 46.069 g/mol
|
10 G_PER_L |
CHEBI:16236
ethanol
|
|
| 8 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Separately filter-sterilize glacial acetic acid and ethanol (use Teflon filters), and aseptically add to the sterile medium. Final pH should be about 3.8.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J891_RAE_MEDIUM (CultureMech:003240)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.917760Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J891 |
| 2026-03-13T06:19:18.847735Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003240 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.728911+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RAE MEDIUM" to "rae_medium" for consistent matching |
| 2026-06-05T07:40:17.857607+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:37.474433+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 993e8d2a1d0396def4c157faf2067f5f3190b0a80e1398ee202177db674ee7b0 |