Overview
- External ontology
- mediadive.medium:J891 (source link unavailable) — RAE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 3.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=891
Composition
flowchart LR
CultureMech_003240["rae_medium"]
ing0_Glucose["Glucose"]
CultureMech_003240 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Peptone["Peptone"]
CultureMech_003240 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_003240 --> ing2_Yeast_extract
ing3_Citric_acid["Citric acid"]
CultureMech_003240 --> ing3_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing3_Citric_acid -.-> CHEBI_30769
ing4_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_003240 --> ing4_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing4_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing5_Glacial_acetic_acid["Glacial acetic acid"]
CultureMech_003240 --> ing5_Glacial_acetic_acid
ing6_Ethanol["Ethanol"]
CultureMech_003240 --> ing6_Ethanol
CHEBI_16236["CHEBI:16236"]
ing6_Ethanol -.-> CHEBI_16236
ing7_Agar["Agar"]
CultureMech_003240 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
40 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Peptone | 10 G_PER_L | ||
| 3 | Yeast extract | 10 G_PER_L | ||
| 4 |
Citric acid
C6H8O7 192.123 g/mol
|
1.5 G_PER_L |
CHEBI:30769
citric acid
|
|
| 5 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
3.38 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 6 | Glacial acetic acid | 10 G_PER_L | ||
| 7 |
Ethanol
C2H6O 46.069 g/mol
|
10 G_PER_L |
CHEBI:16236
ethanol
|
|
| 8 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Separately filter-sterilize glacial acetic acid and ethanol (use Teflon filters), and aseptically add to the sterile medium. Final pH should be about 3.8.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.917760Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J891 |
| 2026-03-13T06:19:18.847735Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003240 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.728911+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RAE MEDIUM" to "rae_medium" for consistent matching |
| 2026-06-05T07:40:17.857607+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T14:52:40.011908+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |