Overview
- External ontology
- mediadive.medium:J89 (source link unavailable) — SM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=89
Composition
flowchart LR
CultureMech_003248["sm_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003248 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4["Na2HPO4"]
CultureMech_003248 --> ing1_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4 -.-> CHEBI_34683
ing2_NH4Cl["NH4Cl"]
CultureMech_003248 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003248 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Na2S2O3["Na2S2O3"]
CultureMech_003248 --> ing4_Na2S2O3
CHEBI_132112["CHEBI:132112"]
ing4_Na2S2O3 -.-> CHEBI_132112
ing5_EDTA["EDTA"]
CultureMech_003248 --> ing5_EDTA
CHEBI_4735["CHEBI:4735"]
ing5_EDTA -.-> CHEBI_4735
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003248 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing7_CaCl2["CaCl2"]
CultureMech_003248 --> ing7_CaCl2
CHEBI_3312["CHEBI:3312"]
ing7_CaCl2 -.-> CHEBI_3312
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003248 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003248 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_003248 --> ing10__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing10__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003248 --> ing11_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003248 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.49254 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
4.47761 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.298507 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0995025 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Na2S2O3
2Na.O3S2 158.111 g/mol
|
100 G_PER_L |
CHEBI:132112
sodium thiosulfate
|
|
| 6 |
EDTA
C10H16N2O8 292.244 g/mol
|
50 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 8 |
CaCl2
Ca.2Cl 110.984 g/mol
|
5.54 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
5.06 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
4.99 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
1.1 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 12 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.57 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.61 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- Adjust pH to 7.5.
- Separately sterilize Na2S2O3 solution by filtration. Aseptically add sterile Na2S2O3 solution to the medium.
- Adjust pH to 6.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sm_medium (CultureMech:003248)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.710751Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J89 |
| 2026-03-13T06:19:18.909266Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003248 |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.799735+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SM MEDIUM" to "sm_medium" for consistent matching |
| 2026-06-05T07:41:45.920839+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.846337+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:11.518150+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.810620+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e3f93a5fa66bc678f3c3397d62f58b9439fa9d194567189cb4560decf49855ef |