sm_medium

CultureMech:003248 bacterial DEFINED LIQUID
Original name: SM MEDIUM

Overview

External ontology
mediadive.medium:J89 (source link unavailable) — SM MEDIUM
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=89

Composition

flowchart LR
    CultureMech_003248["sm_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003248 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_Na2HPO4["Na2HPO4"]
    CultureMech_003248 --> ing1_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4 -.-> CHEBI_34683
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003248 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003248 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Na2S2O3["Na2S2O3"]
    CultureMech_003248 --> ing4_Na2S2O3
    CHEBI_132112["CHEBI:132112"]
    ing4_Na2S2O3 -.-> CHEBI_132112
    ing5_EDTA["EDTA"]
    CultureMech_003248 --> ing5_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing5_EDTA -.-> CHEBI_4735
    ing6_CaCl2["CaCl2"]
    CultureMech_003248 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_003248 --> ing7__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing7__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
1.49254 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 Na2HPO4
HO4P.2Na 141.958 g/mol
4.47761 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.298507 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0995025 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Na2S2O3
2Na.O3S2 158.111 g/mol
100 G_PER_L CHEBI:132112
sodium thiosulfate
6 EDTA
C10H16N2O8 292.244 g/mol
50 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
7 CaCl2
Ca.2Cl 110.984 g/mol
5.54 G_PER_L CHEBI:3312
calcium dichloride
8 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
1.1 G_PER_L CHEBI:91249
ammonium molybdate

Preparation

  1. Adjust pH to 7.5.
  2. Separately sterilize Na2S2O3 solution by filtration. Aseptically add sterile Na2S2O3 solution to the medium.
  3. Adjust pH to 6.0 with KOH.

Solutions

Trace metal solution

Dose: 5 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 22 G_PER_L
MnCl2 x 4 H2O 5.06 G_PER_L
FeSO4 x 7 H2O 4.99 G_PER_L
CuSO4 x 5 H2O 1.57 G_PER_L
CoCl2 x 6 H2O 1.61 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.710751Z mediadive-import Imported from MediaDive Source: JCM, ID: J89
2026-03-13T06:19:18.909266Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003248
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.799735+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SM MEDIUM" to "sm_medium" for consistent matching
2026-06-05T07:41:45.920839+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.846337+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:11.518150+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.485301+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 1 solution(s): 5 -> 'Trace metal solution' @ 5 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).