Overview
- External ontology
- mediadive.medium:J900 (source link unavailable) — M13a MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=900
Composition
flowchart LR
CultureMech_003249["m13a_medium"]
ing0_Peptone["Peptone"]
CultureMech_003249 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003249 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_003249 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_003249 --> ing3_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing3_Tris_HCl_buffer -.-> CHEBI_9754
ing4_Sea_water["Sea water"]
CultureMech_003249 --> ing4_Sea_water
ing5_Vitamin_B12["Vitamin B12"]
CultureMech_003249 --> ing5_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing5_Vitamin_B12 -.-> CHEBI_176843
ing6_Biotin["Biotin"]
CultureMech_003249 --> ing6_Biotin
CHEBI_15956["CHEBI:15956"]
ing6_Biotin -.-> CHEBI_15956
ing7_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_003249 --> ing7_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing7_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing8_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003249 --> ing8_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing8_Calcium_pantothenate -.-> CHEBI_31345
ing9_Folic_acid["Folic acid"]
CultureMech_003249 --> ing9_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing9_Folic_acid -.-> CHEBI_27470
ing10_Riboflavin["Riboflavin"]
CultureMech_003249 --> ing10_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing10_Riboflavin -.-> CHEBI_17015
ing11_Nicotinamide["Nicotinamide"]
CultureMech_003249 --> ing11_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing11_Nicotinamide -.-> CHEBI_17154
ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003249 --> ing12_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003249 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003249 --> ing14_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003249 --> ing15_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003249 --> ing16_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
ing17__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_003249 --> ing17__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing17__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003249 --> ing18_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
ing19_Na2_EDTA["Na2-EDTA"]
CultureMech_003249 --> ing19_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing19_Na2_EDTA -.-> CHEBI_64734
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003249 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003249 --> ing21_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing21_MnSO4_x_n_H2O -.-> CHEBI_86360
ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003249 --> ing22_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
ing23_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_003249 --> ing23_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing23_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
ing24_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_003249 --> ing24_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing24_Na2B4O7_x_10_H2O -.-> CHEBI_131366
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.25 G_PER_L | ||
| 2 | Yeast extract | 0.25 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
50 G_PER_L |
CHEBI:9754
tris
|
|
| 5 | Sea water | 500 G_PER_L | ||
| 6 | Vitamin B12 | 0.0002 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 7 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.004 G_PER_L |
CHEBI:15956
biotin
|
|
| 8 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.01 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 9 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 10 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.001 G_PER_L |
CHEBI:27470
folic acid
|
|
| 11 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 12 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.01 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 13 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 16 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 17 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.335 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 18 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.00925 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 19 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.599 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.099, 0.5] |
| 20 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 22 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.154 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 23 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0392 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 24 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.0248 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
|
| 25 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0177 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Preparation
- For agar plates, increase pH of Tris-HCl buffer to 8.5.
- Filter-sterilize.
- Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- m13a_medium (CultureMech:003249)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.944478Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J900 |
| 2026-03-13T03:09:17.196761Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:18.918593Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003249 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.810007+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M13a MEDIUM" to "m13a_medium" for consistent matching |
| 2026-06-05T07:41:11.664977+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.650617+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:40.745938+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f61e38fe2a881e54e845fd24809f575c01995c4daa9d2378196e9c976a28a1e2 |