m13a_medium

CultureMech:003249 bacterial COMPLEX LIQUID
Original name: M13a MEDIUM

Overview

External ontology
mediadive.medium:J900 (source link unavailable) — M13a MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=900

Composition

flowchart LR
    CultureMech_003249["m13a_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_003249 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003249 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_003249 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_003249 --> ing3_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing3_Tris_HCl_buffer -.-> CHEBI_9754
    ing4_Sea_water["Sea water"]
    CultureMech_003249 --> ing4_Sea_water
    ing5_Vitamin_B12["Vitamin B12"]
    CultureMech_003249 --> ing5_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing5_Vitamin_B12 -.-> CHEBI_176843
    ing6_Biotin["Biotin"]
    CultureMech_003249 --> ing6_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing6_Biotin -.-> CHEBI_15956
    ing7_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_003249 --> ing7_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing7_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing8_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003249 --> ing8_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing8_Calcium_pantothenate -.-> CHEBI_31345
    ing9_Folic_acid["Folic acid"]
    CultureMech_003249 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Riboflavin["Riboflavin"]
    CultureMech_003249 --> ing10_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing10_Riboflavin -.-> CHEBI_17015
    ing11_Nicotinamide["Nicotinamide"]
    CultureMech_003249 --> ing11_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing11_Nicotinamide -.-> CHEBI_17154
    ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003249 --> ing12_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003249 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003249 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003249 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003249 --> ing16_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing17__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_003249 --> ing17__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing17__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003249 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_Na2_EDTA["Na2-EDTA"]
    CultureMech_003249 --> ing19_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing19_Na2_EDTA -.-> CHEBI_64734
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003249 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003249 --> ing21_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing21_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003249 --> ing22_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing23_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_003249 --> ing23_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing23_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing24_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_003249 --> ing24_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing24_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 Peptone 0.25 G_PER_L
2 Yeast extract 0.25 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
0.25 G_PER_L CHEBI:17234
glucose
4 Tris-HCl buffer
C4H11NO3 121.136 g/mol
50 G_PER_L CHEBI:9754
tris
5 Sea water 500 G_PER_L
6 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
7 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
8 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
9 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
10 Folic acid
C19H19N7O6 441.404 g/mol
0.001 G_PER_L CHEBI:27470
folic acid
11 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
12 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
13 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
17 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
18 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
20 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
21 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
22 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.154 G_PER_L CHEBI:86360
manganese(II) sulfate
23 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
24 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
25 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. For agar plates, increase pH of Tris-HCl buffer to 8.5.
  2. Filter-sterilize.
  3. Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.944478Z mediadive-import Imported from MediaDive Source: JCM, ID: J900
2026-03-13T03:09:17.196761Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:18.918593Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003249
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.810007+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M13a MEDIUM" to "m13a_medium" for consistent matching
2026-06-05T07:41:11.664977+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.650617+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.