Overview
- External ontology
- mediadive.medium:J944 (source link unavailable) — MW MEDIUM FOR METHANOSARCINA
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=944
Composition
flowchart LR
CultureMech_003292["mw_medium_for_methanosarcina"]
ing0_NaCl["NaCl"]
CultureMech_003292 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_003292 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003292 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_NH4Cl["NH4Cl"]
CultureMech_003292 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003292 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Resazurin["Resazurin"]
CultureMech_003292 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_KH2PO4["KH2PO4"]
CultureMech_003292 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NaHCO3["NaHCO3"]
CultureMech_003292 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003292 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003292 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003292 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_003292 --> ing11_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing11_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003292 --> ing12_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003292 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003292 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003292 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003292 --> ing16_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003292 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003292 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_H3BO3["H3BO3"]
CultureMech_003292 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003292 --> ing20_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003292 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Nicotinic_acid["Nicotinic acid"]
CultureMech_003292 --> ing22_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing22_Nicotinic_acid -.-> CHEBI_15940
ing23_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_003292 --> ing23_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing23_DL_Calcium_pantothenate -.-> CHEBI_31345
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003292 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Riboflavin["Riboflavin"]
CultureMech_003292 --> ing25_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavin -.-> CHEBI_17015
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_003292 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Biotin["Biotin"]
CultureMech_003292 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_Folic_acid["Folic acid"]
CultureMech_003292 --> ing28_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing28_Folic_acid -.-> CHEBI_27470
ing29_Lipoic_acid["Lipoic acid"]
CultureMech_003292 --> ing29_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29_Lipoic_acid -.-> CHEBI_16494
CultureMech_003292 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.988142 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.494071 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.395257 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.247036 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.148221 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000988142 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
32 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
6 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
6 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 12 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.2 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 13 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.2 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.11 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.25 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.002 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.01 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 24 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 26 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.01 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.005 G_PER_L |
CHEBI:15956
biotin
|
|
| 29 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.005 G_PER_L |
CHEBI:27470
folic acid
|
|
| 30 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 31 | Vitamin B12 | 0.005 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Mix components thoroughly, and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
- Aseptically distribute the medium into culture vessels under a H2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers (leave >80% gas phase by volume). Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
- Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20, v/v).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mw_medium_for_methanosarcina (CultureMech:003292)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.081831Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J944 |
| 2026-01-28T19:48:29.004702Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:19.255207Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003292 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.184249+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MW MEDIUM FOR METHANOSARCINA" to "mw_medium_for_methanosarcina" for consistent matching |
| 2026-06-05T07:41:38.951537+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.314325+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.010925+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1a2a32c7680ef6e11765d570bd3fdd29b940aa9eebc6a53cf823ebfbab2dec34 |