mw_medium_for_methanosarcina

CultureMech:003292 archaea DEFINED LIQUID
Original name: MW MEDIUM FOR METHANOSARCINA

Overview

External ontology
mediadive.medium:J944 (source link unavailable) — MW MEDIUM FOR METHANOSARCINA
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=944

Composition

flowchart LR
    CultureMech_003292["mw_medium_for_methanosarcina"]
    ing0_NaCl["NaCl"]
    CultureMech_003292 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_003292 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003292 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_NH4Cl["NH4Cl"]
    CultureMech_003292 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003292 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Resazurin["Resazurin"]
    CultureMech_003292 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_KH2PO4["KH2PO4"]
    CultureMech_003292 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_NaHCO3["NaHCO3"]
    CultureMech_003292 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003292 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003292 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003292 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_003292 --> ing11_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing11_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003292 --> ing12_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003292 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003292 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003292 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003292 --> ing16_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003292 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003292 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_H3BO3["H3BO3"]
    CultureMech_003292 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003292 --> ing20_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003292 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003292 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_003292 --> ing23_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003292 --> ing24_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing25_Riboflavin["Riboflavin"]
    CultureMech_003292 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003292 --> ing26_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing26_Thiamine_HCl -.-> CHEBI_49105
    ing27_Biotin["Biotin"]
    CultureMech_003292 --> ing27_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing27_Biotin -.-> CHEBI_15956
    ing28_Folic_acid["Folic acid"]
    CultureMech_003292 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470
    ing29_Lipoic_acid["Lipoic acid"]
    CultureMech_003292 --> ing29_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29_Lipoic_acid -.-> CHEBI_16494
    CultureMech_003292 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
0.988142 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.494071 G_PER_L CHEBI:32588
potassium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.395257 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.247036 G_PER_L CHEBI:31206
ammonium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.148221 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 Resazurin
C12H7NO4 229.191 g/mol
0.000988142 G_PER_L CHEBI:8806
Resazurin
7 KH2PO4
H2O4P.K 136.084 g/mol
2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
8 NaHCO3
CHO3.Na 84.006 g/mol
32 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
6 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
12 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.2 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
13 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.2 G_PER_L CHEBI:131361
disodium selenite pentahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.11 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.1 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.25 G_PER_L CHEBI:34887
nickel dichloride
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.2 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.002 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
23 Nicotinic acid
C6H5NO2 123.111 g/mol
0.01 G_PER_L CHEBI:15940
nicotinic acid
24 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
25 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
27 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.01 G_PER_L CHEBI:49105
thiamine hydrochloride
28 Biotin
C10H16N2O3S 244.316 g/mol
0.005 G_PER_L CHEBI:15956
biotin
29 Folic acid
C19H19N7O6 441.404 g/mol
0.005 G_PER_L CHEBI:27470
folic acid
30 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
31 Vitamin B12 0.005 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Mix components thoroughly, and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
  2. Aseptically distribute the medium into culture vessels under a H2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers (leave >80% gas phase by volume). Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
  3. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20, v/v).
  4. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.081831Z mediadive-import Imported from MediaDive Source: JCM, ID: J944
2026-01-28T19:48:29.004702Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:19.255207Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003292
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.184249+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MW MEDIUM FOR METHANOSARCINA" to "mw_medium_for_methanosarcina" for consistent matching
2026-06-05T07:41:38.951537+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.314325+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.