modified_growth_medium_with_23_total_salt_concentration

CultureMech:003304 bacterial COMPLEX LIQUID
Original name: MODIFIED GROWTH MEDIUM WITH 23% TOTAL SALT CONCENTRATION

Overview

External ontology
mediadive.medium:J956 (source link unavailable) — MODIFIED GROWTH MEDIUM WITH 23% TOTAL SALT CONCENTRATION
Applications
  • Microbial cultivation
Synonyms
  • {'name': '5_salt_water_growth_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J897', 'original_category': 'bacterial'}
pH
7.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=956

Composition

flowchart LR
    CultureMech_003304["modified_growth_medium_with_23_total_salt_concentration"]
    ing0_Peptone["Peptone"]
    CultureMech_003304 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003304 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_003304 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003304 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003304 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KCl["KCl"]
    CultureMech_003304 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2["CaCl2"]
    CultureMech_003304 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L
2 Yeast extract 1 G_PER_L
3 NaCl
Cl.Na 58.443 g/mol
240 G_PER_L CHEBI:26710
sodium chloride
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
30 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
35 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 KCl
Cl.K 74.551 g/mol
7 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2
Ca.2Cl 110.984 g/mol
0.5549 G_PER_L CHEBI:3312
calcium dichloride

Preparation

  1. Mix components thoroughly and adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar.
  2. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:18.127462Z mediadive-import Imported from MediaDive Source: JCM, ID: J956
2026-03-13T06:19:19.349255Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003304
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.291708+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED GROWTH MEDIUM WITH 23% TOTAL SALT CONCENTRATION" to "modified_growth_medium_with_23_total_salt_concentration" for consistent matching
2026-06-05T07:41:36.619872+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:04:34.158202+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: 5_salt_water_growth_medium, modified_growth_medium_with_23_total_salt_concentration
2026-08-06T03:04:34.158233+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 6cdbfca0f2a3912315699291ff8757cc1352d56579a85c6bf3a927e255406ff4