Overview
- External ontology
- mediadive.medium:J956 (source link unavailable) — MODIFIED GROWTH MEDIUM WITH 23% TOTAL SALT CONCENTRATION
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=956
Composition
flowchart LR
CultureMech_003304["modified_growth_medium_with_23_total_salt_concentration"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003304 --> ing0_Yeast_extract
ing1_Peptone["Peptone"]
CultureMech_003304 --> ing1_Peptone
ing2_NaCl["NaCl"]
CultureMech_003304 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003304 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003304 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_KCl["KCl"]
CultureMech_003304 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_CaCl2["CaCl2"]
CultureMech_003304 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 | Peptone | 10 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
240 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
30 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
35 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
7 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.5549 G_PER_L |
CHEBI:3312
calcium dichloride
|
Preparation
- Mix components thoroughly and adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar.
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.127462Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J956 |
| 2026-03-13T06:19:19.349255Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003304 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.291708+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED GROWTH MEDIUM WITH 23% TOTAL SALT CONCENTRATION" to "modified_growth_medium_with_23_total_salt_concentration" for consistent matching |
| 2026-06-05T07:41:36.619872+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |