Overview
- External ontology
- mediadive.medium:J958 (source link unavailable) — SEA SALTS TYG MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=958
Composition
flowchart LR
CultureMech_003306["sea_salts_tyg_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_003306 --> ing0_Sea_Salt
ing1_Trypticase_peptone["Trypticase peptone"]
CultureMech_003306 --> ing1_Trypticase_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_003306 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_003306 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Resazurin["Resazurin"]
CultureMech_003306 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Na2CO3["Na2CO3"]
CultureMech_003306 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003306 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003306 --> ing7_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003306 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003306 --> ing9_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing9_MnSO4_x_n_H2O -.-> CHEBI_86360
ing10_NaCl["NaCl"]
CultureMech_003306 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003306 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003306 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003306 --> ing13_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003306 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003306 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_003306 --> ing16_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing17_H3BO3["H3BO3"]
CultureMech_003306 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003306 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_003306 --> ing19__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing19__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003306 --> ing20_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing21_Na2SeO4["Na2SeO4"]
CultureMech_003306 --> ing21_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing21_Na2SeO4 -.-> CHEBI_77775
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 49.3097 G_PER_L | ||
| 2 | Trypticase peptone | 0.986193 G_PER_L | ||
| 3 | Yeast extract | 0.986193 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.986193 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000493097 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.986193 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
6 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
6 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
30 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.8 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 17 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 21 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 22 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
Preparation
- Mix components thoroughly and autoclave under a N2-CO2 gas mixture. After cooling, adjust pH to 7.2 - 7.5 with 5% Na2CO3 solution (autoclaved and stored under N2). Then, add per litter the following solutions (autoclaved and stored under N2):
- First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sea_salts_tyg_medium (CultureMech:003306)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.134071Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J958 |
| 2026-03-13T06:19:19.370658Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003306 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.316491+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SEA SALTS TYG MEDIUM" to "sea_salts_tyg_medium" for consistent matching |
| 2026-06-05T07:41:45.421215+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.811667+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:11.351910+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.418822+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6486dbac7e98f4079465bf706bf4b0ca0f86c40cf91a4e1cf2093f86e5d60346 |