sea_salts_tyg_medium

CultureMech:003306 bacterial COMPLEX LIQUID
Original name: SEA SALTS TYG MEDIUM

Overview

External ontology
mediadive.medium:J958 (source link unavailable) — SEA SALTS TYG MEDIUM
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=958

Composition

flowchart LR
    CultureMech_003306["sea_salts_tyg_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_003306 --> ing0_Sea_Salt
    ing1_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003306 --> ing1_Trypticase_peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_003306 --> ing2_Yeast_extract
    ing3_Glucose["Glucose"]
    CultureMech_003306 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Resazurin["Resazurin"]
    CultureMech_003306 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Na2CO3["Na2CO3"]
    CultureMech_003306 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003306 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003306 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003306 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_NaCl["NaCl"]
    CultureMech_003306 --> ing9_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing9_NaCl -.-> CHEBI_26710
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003306 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003306 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_003306 --> ing12_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing12_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing13_H3BO3["H3BO3"]
    CultureMech_003306 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003306 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_003306 --> ing15__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing15__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003306 --> ing16_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing17_Na2SeO4["Na2SeO4"]
    CultureMech_003306 --> ing17_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing17_Na2SeO4 -.-> CHEBI_77775

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Sea Salt 49.3097 G_PER_L
2 Trypticase peptone 0.986193 G_PER_L
3 Yeast extract 0.986193 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
0.986193 G_PER_L CHEBI:17234
glucose
5 Resazurin
C12H7NO4 229.191 g/mol
0.000493097 G_PER_L CHEBI:8806
Resazurin
6 Na2CO3
CO3.2Na 105.988 g/mol
0.986193 G_PER_L CHEBI:29377
sodium carbonate
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
6 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
30 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
10 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
13 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.18 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2.8 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
17 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
18 Na2SeO4
2Na.O4Se 188.936 g/mol
0.1 G_PER_L CHEBI:77775
sodium selenate

Preparation

  1. Mix components thoroughly and autoclave under a N2-CO2 gas mixture. After cooling, adjust pH to 7.2 - 7.5 with 5% Na2CO3 solution (autoclaved and stored under N2). Then, add per litter the following solutions (autoclaved and stored under N2):
  2. First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.

Solutions

Wolfe's mineral elixir

Dose: 2 ML_PER_L

Stock composition
IngredientConcentrationNotes
MnSO4 x n H2O 5 G_PER_L
FeSO4 x 7 H2O 1 G_PER_L
CoCl2 x 6 H2O 1.8 G_PER_L
ZnSO4 x 7 H2O 1.8 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.134071Z mediadive-import Imported from MediaDive Source: JCM, ID: J958
2026-03-13T06:19:19.370658Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003306
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.316491+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SEA SALTS TYG MEDIUM" to "sea_salts_tyg_medium" for consistent matching
2026-06-05T07:41:45.421215+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.811667+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:11.351910+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.473829+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> "Wolfe's mineral elixir" @ 2 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).