acidifferobacter_murciensis_medium

CultureMech:003319 bacterial DEFINED LIQUID
Original name: ACIDIFFEROBACTER MURCIENSIS MEDIUM

Overview

External ontology
mediadive.medium:J970 (source link unavailable) — ACIDIFFEROBACTER MURCIENSIS MEDIUM
Applications
  • Microbial cultivation
pH
2.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=970

Composition

flowchart LR
    CultureMech_003319["acidifferobacter_murciensis_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003319 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_003319 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003319 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003319 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003319 --> ing4_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing5_ZnCl2["ZnCl2"]
    CultureMech_003319 --> ing5_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing5_ZnCl2 -.-> CHEBI_49976
    ing6_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003319 --> ing6_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing6_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing7_H3BO3["H3BO3"]
    CultureMech_003319 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003319 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003319 --> ing9_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing9_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003319 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_H2SO4["H2SO4"]
    CultureMech_003319 --> ing11_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing11_H2SO4 -.-> CHEBI_26836

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.131936 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.0271457 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.0253493 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.146707 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
120 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
6 ZnCl2
2Cl.Zn 136.296 g/mol
70 G_PER_L CHEBI:49976
zinc dichloride
7 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
120 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
8 H3BO3
H3BO3 61.833 g/mol
31 G_PER_L CHEBI:33118
boric acid
9 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
12.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
10 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
85.2 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
11 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
19.9601 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
12 H2SO4
H2O4S 98.079 g/mol
2 G_PER_L CHEBI:26836
sulfuric acid

Preparation

  1. Adjust pH to 2.0 with 10 N H2SO4 and autoclave. After autoclaving, add the following solution (separately autoclaved):
  2. <I>Iron(II) sulfate solution: </I>

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.170323Z mediadive-import Imported from MediaDive Source: JCM, ID: J970
2026-03-13T06:19:19.462310Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003319
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.425779+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIFFEROBACTER MURCIENSIS MEDIUM" to "acidifferobacter_murciensis_medium" for consistent matching
2026-06-05T07:40:47.199152+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.557890+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:21.809391+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 180780154e74290000cf4c81172ae021b1a8ca17ab032b691dd84b7455c0c486