Overview
- External ontology
- mediadive.medium:J970 (source link unavailable) — ACIDIFFEROBACTER MURCIENSIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 2.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=970
Composition
flowchart LR
CultureMech_003319["acidifferobacter_murciensis_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_003319 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_003319 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003319 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003319 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003319 --> ing4_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
ing5_ZnCl2["ZnCl2"]
CultureMech_003319 --> ing5_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing5_ZnCl2 -.-> CHEBI_49976
ing6_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003319 --> ing6_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing6_CoCl2_x_6_H2O -.-> CHEBI_53503
ing7_H3BO3["H3BO3"]
CultureMech_003319 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003319 --> ing8_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing9_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003319 --> ing9_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing9_CuCl2_x_2_H2O -.-> CHEBI_86318
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003319 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_H2SO4["H2SO4"]
CultureMech_003319 --> ing11_H2SO4
CHEBI_26836["CHEBI:26836"]
ing11_H2SO4 -.-> CHEBI_26836
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.131936 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0271457 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0253493 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.146707 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
120 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 6 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 7 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
120 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
31 G_PER_L |
CHEBI:33118
boric acid
|
|
| 9 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
12.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 10 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
85.2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
19.9601 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
H2SO4
H2O4S 98.079 g/mol
|
2 G_PER_L |
CHEBI:26836
sulfuric acid
|
Preparation
- Adjust pH to 2.0 with 10 N H2SO4 and autoclave. After autoclaving, add the following solution (separately autoclaved):
- <I>Iron(II) sulfate solution: </I>
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.170323Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J970 |
| 2026-03-13T06:19:19.462310Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003319 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.425779+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDIFFEROBACTER MURCIENSIS MEDIUM" to "acidifferobacter_murciensis_medium" for consistent matching |
| 2026-06-05T07:40:47.199152+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.557890+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |