Overview
- External ontology
- mediadive.medium:J973 (source link unavailable) — HALORHABDUS SEGORENSIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=973
Composition
flowchart LR
CultureMech_003322["halorhabdus_segorensis_medium"]
ing0_NaCl["NaCl"]
CultureMech_003322 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003322 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003322 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_003322 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Yeast_extract["Yeast extract"]
CultureMech_003322 --> ing4_Yeast_extract
ing5_Casamino_acids["Casamino acids"]
CultureMech_003322 --> ing5_Casamino_acids
ing6_Starch["Starch"]
CultureMech_003322 --> ing6_Starch
CHEBI_28017["CHEBI:28017"]
ing6_Starch -.-> CHEBI_28017
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
40.1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
203 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
29.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
7.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 | Yeast extract | 1 G_PER_L | ||
| 6 | Casamino acids | 1 G_PER_L | ||
| 7 | Starch | 2 G_PER_L |
CHEBI:28017
starch
|
Preparation
- Dissolve MgCl2 x 6H2O in distilled water and bring volume to 500.0 ml. Separately dissolve the remaining components in 500 ml distilled water and adjust pH to 7.0. For preparation of solid medium, add 20.0 g agar to the latter solution. Autoclave the two solutions separately and combine them after autoclaving.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- halorhabdus_segorensis_medium (CultureMech:003322)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.180122Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J973 |
| 2026-01-28T19:48:10.304318Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:19.486702Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003322 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.490831+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALORHABDUS SEGORENSIS MEDIUM" to "halorhabdus_segorensis_medium" for consistent matching |
| 2026-06-05T07:41:08.108887+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.774612+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:04:28.904096+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 70dc0d931a6de801ac303740c4dd696fda7dc82ea97b607db5f9283791e8bd58 |