Overview
- External ontology
- mediadive.medium:J973 (source link unavailable) — HALORHABDUS SEGORENSIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=973
Ingredients (0)
No ingredients recorded.
Preparation
- Dissolve MgCl2 x 6H2O in distilled water and bring volume to 500.0 ml. Separately dissolve the remaining components in 500 ml distilled water and adjust pH to 7.0. For preparation of solid medium, add 20.0 g agar to the latter solution. Autoclave the two solutions separately and combine them after autoclaving.
Solutions
JCM973 magnesium chloride preparation
| Ingredient | Concentration | Notes |
|---|---|---|
| MgCl2 x 6 H2O | 406 G_PER_L | Separate magnesium chloride hexahydrate stock before autoclaving: 203 g / (500.0 ml / 1000 ml per L) = 406 g/L. The former flattened 203 g/L was not a verified final-medium concentration. No final-medium concentration is calculated. |
| Distilled water | Distilled water is added as needed to make the magnesium stock up to 500.0 ml. This is a stock final-volume instruction, not 500 ml of water input. Its water amount remains unquantified. |
Use the complete magnesium stock batch prepared to 0.5 L before autoclaving. This is its prepared batch volume, not a measured post-autoclave volume, working dose per liter, or final-medium volume. The actual volume added after autoclaving is unreported; no numeric working dose is asserted.
JCM973 remaining-components preparation
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 0.5 L | Source input for the remaining-components preparation: 500 ml distilled water / 1000 ml per L = 0.5 L. This is not its final solution volume or the final-medium volume. |
| NaCl | Source amount: 40.1 g per remaining-components recipe batch, not per liter. The 500 ml quantity is water input, not final stock volume; the final mixed-medium volume is unreported. The former G_PER_L encoding is withdrawn. | |
| CaCl2 x 2 H2O | Source amount: 29.4 g per remaining-components recipe batch, not per liter. The 500 ml quantity is water input, not final stock volume; the final mixed-medium volume is unreported. The former G_PER_L encoding is withdrawn. | |
| KCl | Source amount: 7.5 g per remaining-components recipe batch, not per liter. The 500 ml quantity is water input, not final stock volume; the final mixed-medium volume is unreported. The former G_PER_L encoding is withdrawn. | |
| Yeast extract | Source amount: 1 g per remaining-components recipe batch, not per liter. The 500 ml quantity is water input, not final stock volume; the final mixed-medium volume is unreported. The former G_PER_L encoding is withdrawn. Source material attribute: BD-Difco. | |
| Casamino acids | Source amount: 1 g per remaining-components recipe batch, not per liter. The 500 ml quantity is water input, not final stock volume; the final mixed-medium volume is unreported. The former G_PER_L encoding is withdrawn. Source material attribute: BD-Difco. | |
| Starch | Source amount: 2 g per remaining-components recipe batch, not per liter. The 500 ml quantity is water input, not final stock volume; the final mixed-medium volume is unreported. The former G_PER_L encoding is withdrawn. Source material attribute: soluble. |
Use the complete separately prepared remaining-components batch. The source specifies 500 ml water input, not a 500 ml final solution volume; no numeric stock working dose or final-medium concentration is inferred.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.180122Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J973 |
| 2026-01-28T19:48:10.304318Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:19.486702Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003322 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.490831+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALORHABDUS SEGORENSIS MEDIUM" to "halorhabdus_segorensis_medium" for consistent matching |
| 2026-06-05T07:41:08.108887+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.774612+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-08T10:03:37.542180+00:00 | codex | RESTORED_JCM973_PREPARATION_STAGES_WITH_DATASET_EVIDENCE | Restored two separately prepared JCM973 components from inspected dataset instructions. Rebased magnesium to its explicit 500 ml stock; withdrew unsupported mass-per-liter encodings while preserving exact batch masses. Kept makeup water unquantified and 500 ml base water separate. No final-medium volume, ontology IDs, physical-state changes or organism claims inferred. Historical provenance unchanged. |